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  1. 広島大学博士論文
  2. 2012年度以前

無尾両生類を用いた発生異常の研究

https://hiroshima.repo.nii.ac.jp/records/2005453
https://hiroshima.repo.nii.ac.jp/records/2005453
f646f2f1-4457-490c-b942-1307059b50f7
名前 / ファイル ライセンス アクション
diss_otsu3913.pdf diss_otsu3913.pdf (2.8 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Investigations of abnormal development using anurans
言語 en
タイトル
タイトル 無尾両生類を用いた発生異常の研究
言語 ja
作成者 田澤, 一朗

× 田澤, 一朗

ja 田澤, 一朗

en Tazawa, Ichiro

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright(c) by Author
主題
主題Scheme NDC
主題 480
内容記述
内容記述 Developing oocytes arrest at the prophase of meiosis I, while the hormone progesterone restarts the process, leading to full maturation as fertile eggs. In this study we investigated the effects of hypergravity on this phase of maturation using oocytes of Xenopus. No differences were found between 2G and 5G treated groups and untreated controls regarding time to resumption of maturation, as judged by the appearance of a white spot in the animal hemisphere. However the white spot itself was larger and brighter in treated oocytes at time of appearance, and became even more pronounced as maturation proceeded. These findings suggest that although hypergravity does not affect the resumption of maturation, it might adversely affect the subsequent process and the final results. Because there is mounting evidence to suggest that oxidative stress is involved in the pathophysiology of albinism, albino amphibians are useful tools for studies on imbalances in the oxidant-antioxidant system. In the course of maintaining albino mutant frog strains it was found that crosses between albino males and heterozygous females of Rana nigromaculata sometimes produce offspring displaying pigmentary mosaicism. After hatching hypopigmented portions appear on the left or right side of the body, and this is accompanied by such abnormalities as poor viability, asymmetrical curvature of the body toward the hypopigmented side, and limb deformity. Histological examination of mosaics showed the cells of various tissues (except kidney) to be smaller on the hypopigmented side and larger on the pigmented side compared to corresponding cells in wild type offspring. Cytogenetic analysis of cultured skin cells revealed that wild type and albino individuals were diploidal with 26 chromosomes, the same as normal R. nigromaculata. In mosaics on the other hand, cells of hypopigmented portions were almost exclusively haploidal with 13 chromosomes, while pigmented portions were a mixture of roughly 75% triploidal, 39 chromosome cells and roughly 25% haploidal, 13 chromosome cells.
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_db06
資源タイプ doctoral thesis
関連情報
関連タイプ references
関連名称 Effects of hypergravity on oocyte maturation in Xenopus laevis. Ichiro Tazawa, Akihiko Kashiwagi, Keiko Kashiwagi, and Nobuaki Furuno. Biol. Sci. Space, 19 (4) (2005) 242-244.
関連情報
関連タイプ references
関連名称 Skin pigmentary variants in Rana nigromaculata. Ichiro Tazawa, Hitoshi Okumoto, and Akihiko Kashiwagi. J. Clin. Biochem. Nutr., 38 (3) (2006) 195-203.
関連情報
関連タイプ references
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.2187/bss.19.242
関連情報
関連タイプ references
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.3164/jcbn.38.195
学位授与番号
学位授与番号 乙第3913号
学位名
言語 ja
学位名 博士(理学)
学位名
言語 en
学位名 Physical Science
学位授与年月日
学位授与年月日 2006-03-06
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 15401
言語 ja
学位授与機関名 広島大学
学位授与機関
言語 en
学位授与機関名 Hiroshima University
旧ID 21578
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