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酸化ストレスによる網膜色素上皮細胞内の接着蛋白質claudin-1の発現変化
https://hiroshima.repo.nii.ac.jp/records/2005056
https://hiroshima.repo.nii.ac.jp/records/2005056392bf3b1-539d-48ce-97fc-7100254610b6
| 名前 / ファイル | ライセンス | アクション |
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k6272_3.pdf (527.5 KB)
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k6272_1.pdf (275.9 KB)
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k6272_2.pdf (176.1 KB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||||
| タイトル | ||||||||||
| タイトル | Oxidative stress regulates expression of claudin-1 in human RPE cells | |||||||||
| 言語 | en | |||||||||
| タイトル | ||||||||||
| タイトル | 酸化ストレスによる網膜色素上皮細胞内の接着蛋白質claudin-1の発現変化 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
平田, 潤子
× 平田, 潤子
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Age-related macular degeneration | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Retinal pigment epithelium | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Oxidative stress | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Claudin-1 | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Tight junction | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | p38 | |||||||||
| 主題 | ||||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Mitogen-activated protein kinase | |||||||||
| 主題 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 490 | |||||||||
| 内容記述 | ||||||||||
| 内容記述 | Age-related macular degeneration (AMD) is a neurodegenerative disease associated with irreversible loss of central vision in the elderly. Disruption of the homeostatic function of the retinal pigment epithelium (RPE) is thought to be fundamental to AMD pathogenesis, and oxidative stress is implicated in the associated RPE damage. We examined the effects of oxidative stress on the expression of junctional proteins in cultured human retinal pigment epithelial (ARPE-19) cells. Reverse transcription-PCR and immunoblot analyses revealed that expression of the tight-junction protein claudin-1 was increased at both the mRNA and protein levels 8 to 12 h after exposure of ARPE-19 cells to H2O2, whereas that of the tight-junction protein ZO-1 or the adherens-junction protein N-cadherin was unaffected. Expression of both claudin-1 and N-cadherin was down-regulated by exposure of the cells to H2O2 for longer periods (24 to 48 h). Oxidative stress also induced the phosphorylation of p38 mitogen-activated protein kinase (MAPK) with a time course similar to that apparent for the up-regulation of claudin-1 expression. Furthermore, the increase in the abundance of claudin-1 induced by H2O2 was blocked by the p38 inhibitor SB203580. Phosphorylation of the MAPKs ERK and JNK was not affected by H2O2. Our results suggest that modulation of claudin-1 expression in the RPE by oxidative stress may contribute to the pathogenesis of AMD. | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | NA | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||
| 関連情報 | ||||||||||
| 関連タイプ | references | |||||||||
| 関連名称 | Junko Hirata, Ji-Ae Ko, Hideki Mochizuki, Kunihiko Funaishi, Ken Yamane, Koh-Hei Sonoda and Yoshiaki Kiuchi; Oxidative stress regulates expression of claudin-1 in human RPE cells; Central European Journal of Biology, May 2014, Volume 9, Issue 5, pp 461-468 (doi: 10.2478/s11535-014-0287-0) | |||||||||
| 関連情報 | ||||||||||
| 関連タイプ | references | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | http://doi.org/10.2478/s11535-014-0287-0 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第6272号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士(医学) | |||||||||
| 学位名 | ||||||||||
| 言語 | en | |||||||||
| 学位名 | Philosophy in Medical Science | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2013-11-28 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 15401 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 広島大学 | |||||||||
| 学位授与機関 | ||||||||||
| 言語 | en | |||||||||
| 学位授与機関名 | Hiroshima University | |||||||||
| 旧ID | 40614 | |||||||||
