WEKO3
アイテム
シスプラチンとポリ(ADP-リボース)ポリメラーゼ阻害の併用効果には相同組換え修復機構が関与する
https://hiroshima.repo.nii.ac.jp/records/2005053
https://hiroshima.repo.nii.ac.jp/records/2005053d38c73b7-baa2-4a37-a1e3-ec09a379c62e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
k6269_3.pdf (914.9 KB)
|
||
|
k6269_1.pdf (176.9 KB)
|
||
|
k6269_2.pdf (178.5 KB)
|
| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2014-11-21 | |||||||||
| タイトル | ||||||||||
| タイトル | Involvement of homologous recombination in the synergism between cisplatin and poly (ADP-ribose) polymerase inhibition | |||||||||
| 言語 | en | |||||||||
| タイトル | ||||||||||
| タイトル | シスプラチンとポリ(ADP-リボース)ポリメラーゼ阻害の併用効果には相同組換え修復機構が関与する | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
迫川, 賢士
× 迫川, 賢士
|
|||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 権利情報 | ||||||||||
| 権利情報 | Copyright(c) by Author | |||||||||
| 主題 | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 490 | |||||||||
| 内容記述 | ||||||||||
| 内容記述 | Poly (ADP-ribose) polymerase (PARP) plays a critical role in responding to DNA damage, by activating DNA repair pathways responsible for cellular survival. Inhibition of PARP is used to treat certain solid cancers, such as breast and ovarian cancers. However, its effectiveness with other solid cancers, such as esophageal squamous cell carcinoma (ESCC), has not been clarified. We evaluated the effects of PARP inhibition on the survival of human esophageal cancer cells, with a special focus on the induction and repair of DNA double-strand breaks. The effects were monitored by colony formation assays and DNA damage responses, with immunofluorescence staining of γH2AX and RAD51. We found that PARP inhibition synergized with cisplatin, and the cells were highly sensitive, in a similar manner to the combination of cisplatin and 5-fluorouracil (5-FU). Comparable increases in RAD51 foci formation were observed after each combined treatment with cisplatin and either 3-aminobenzamide (3-AB) or 5-FU in three human esophageal cancer cell lines, TE11, TE14, and TE15. In addition, decreasing the amount of RAD51 by RNA interference rendered the TE11 cells even more hypersensitive to these treatments. Our findings suggested that the homologous recombinational repair pathway may be involved in the synergism between cisplatin and either 3-AB or 5-FU, and that 3-AB and 5-FU may similarly modify the cisplatin-induced DNA damage to types requiring the recruitment of RAD51 proteins for their repair. Understanding these mechanisms could be useful for improving the clinical outcome of ESCC patients who suffer from aggressive disease that presently lacks effective treatment options. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2014-11-21 | |||||||||
| 日付タイプ | Created | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | NA | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||
| 関連情報 | ||||||||||
| 関連タイプ | references | |||||||||
| 関連名称 | Kenji Sakogawa, Yoshiro Aoki, Keizo Misumi, Yoichi Hamai, Manabu Emi, Jun Hihara, Lin Shi, Kazuteru Kono, Yasunori Horikoshi, Jiying Sun, Tsuyoshi Ikura, Morihito Okada and Satoshi Tashiro; Involvement of homologous recombination in the synergism between cisplatin and poly (ADP-ribose) polymerase inhibition; Cancer Science, Volume 104, Issue 12, pages 1593-1599, December 2013 (doi: 10.1111/cas.12281) | |||||||||
| 関連情報 | ||||||||||
| 関連タイプ | references | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | http://dx.doi.org/10.1111/cas.12281 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第6269号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士(医学) | |||||||||
| 学位名 | ||||||||||
| 言語 | en | |||||||||
| 学位名 | Philosophy in Medical Science | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2013-11-28 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 15401 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 広島大学 | |||||||||
| 学位授与機関 | ||||||||||
| 言語 | en | |||||||||
| 学位授与機関名 | Hiroshima University | |||||||||
| 旧ID | 36278 | |||||||||
