| Item type |
デフォルト(1) |
| 公開日 |
2025-10-14 |
| タイトル |
|
|
タイトル |
Performance of high-throughput microfluidic real-time qPCR technology for the simultaneous detection of six Alexandrium species in environmental samples |
|
言語 |
en |
| 作成者 |
Cucchi, Francesca
Clarke, Dave
Kavanagh, Fiona
Curtin, Amanda
Caputo, Nicolè
Butler, Francis
Mirimin, Luca
|
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Imai, Ichiro |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Matsushima, Ryoji |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nagai, Satoshi |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nishitani, Goh |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Sakamoto, Setsuko |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Suzuki, Toshiyuki |
|
|
言語 |
en |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Alexandrium |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
HT-qPCR |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Paralytic Shellfish Toxins |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
BiomarkHD |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Surveillance and Management |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Several species of the genus Alexandrium are associated with the production of Paralytic Shellfish Toxins (PSTs) causing the human illness known as Paralytic Shellfish Poisoning (PSP) through the consumption of contaminated filter-feeding molluscs. The threat to public health and the severe economic impact on shellfish industries that PST events constitute urge the development of rapid, efficient, and reliable monitoring tools. High-throughput qPCR systems such as the Standard BioTools’ BiomarkHD have the potential to perform up to 9,216 reactions simultaneously, providing significant cost and time advantages. This study investigated the transferability of six previously published qPCR assays to the BiomarkHD real-time HT-qPCR system, for the simultaneous DNA detection of Alexandrium minutum, A. tamarense, A. catenella, A. mediterraneum, A. tamutum, and A. ostenfeldii. Double-stranded synthetic DNA fragments (dsDNA) and environmental samples were used to evaluate the platform performances in terms of efficiency of amplification (E), Limits of Detection (LOD), Limits of Quantification (LOQ), linearity (r2), dynamic range (DR), inhibition control, and taxonomical identification reliability. For dsDNA, BiomarkHD results were overall comparable to conventional qPCR, while on environmental samples the instrument showed better inhibition control. On the side of taxonomical identification, both platforms confirmed Alexandrium minutum as the species present in all the field samples analyzed. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
本文は、ISSHAのHP（ リンク：https://issha.org/publications-resources/conference-proceedings/ ）
にて公開。 |
|
言語 |
ja |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
The PSPSafe Project is supported and funded by the Irish Department of Agriculture, Food and the Marine (DAFM, Project Ref 2021R578). |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Ichiro Imai, Ryoji Matsushima, Satoshi Nagai, Goh Nishitani, Setsuko Sakamoto, Toshiyuki Suzuki (Eds.). 2023. Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan. International Society for the Study of Harmful Algae. 【121-124】 pp. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
International Society for the Study of Harmful Algae |
|
言語 |
en |
| 日付 |
|
|
日付 |
2025-10-24 |
|
日付タイプ |
Available |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_5794 |
|
資源タイプ |
conference paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| ID登録 |
|
|
ID登録 |
10.15027/0002041282 |
|
ID登録タイプ |
JaLC |
| 書誌情報 |
en : Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan
巻 20,
p. 121-124,
ページ数 4,
発行日 2025-10-24
|
| 見出し |
|
|
大見出し |
Surveillance and Management |
|
言語 |
en |
27(pp.121-124).pdf (479 KB)
