| Item type |
デフォルト(1) |
| 公開日 |
2025-10-14 |
| タイトル |
|
|
タイトル |
The magical mystery tour that is the dinoflagellate sterolysin biosynthesis some assembly (correction) required |
|
言語 |
en |
| 作成者 |
Place, Allen R.
Williams, Ernest P
Haq, Saddef
Bachvaroff, Tsevtan R.
|
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Imai, Ichiro |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Matsushima, Ryoji |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nagai, Satoshi |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nishitani, Goh |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Sakamoto, Setsuko |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Suzuki, Toshiyuki |
|
|
言語 |
en |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Dinoflagellate |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PKS |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Fatty acids |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cerulenin |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Transcript |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We have uncovered a single transcript (>14 Kb) with three consecutive KS modules, that are conserved across three species of dinoflagellates (Karlodinium veneficum and Amphidinium carterae, have known toxins, and Akashiwo sanguinea, which is non-toxic), as well as many single module KS subunits using RNA sequencing analysis. When comparing the three species, the acyl transferase (AT) subunit in the triple module KS was present only in the non-toxic species and missing in the toxin producing species. To test the functionality of PKS in these species, we added cerulenin, an inhibitor that covalently binds to the KS subunits (Funabashi et al, 1989), to an exponential phase culture of A. carterae and A. sanguinea. Using 14C labeled acetate and liquid chromatography mass spectrometry (LC/MS), we found that cerulenin inhibition resulted in the reduction of both fatty acid and toxin production. This shows KS plays a role in both fat and toxin synthesis leading us to our hypothesis: The triple module KS acts as a scaffold for both toxin and fatty acid production where the final product is mediated by accessory trans-AT subunits, which exist in parallel with the KS. To substantiate that the triple KS transcript produces a protein, we have generated an antibody to an epitope in the TE domain. We clearly have evidence that a partial multi-module protein (~285,000 daltons) is expressed and is predicted to catalyze two carbon additions to a growing fatty acid/polyketide chain. However, an antibody to KS1 domain found a discrete protein containing the KS1 and KS2 domains but not the KS3 domain indicating post-translational processing. We are currently investigating the interacting partners with these two protein scaffolds. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
本文は、ISSHAのHP（ リンク：https://issha.org/publications-resources/conference-proceedings/ ）にて公開。 |
|
言語 |
ja |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Ichiro Imai, Ryoji Matsushima, Satoshi Nagai, Goh Nishitani, Setsuko Sakamoto, Toshiyuki Suzuki (Eds.). 2023. Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan. International Society for the Study of Harmful Algae. 【97-100】 pp. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
International Society for the Study of Harmful Algae |
|
言語 |
en |
| 日付 |
|
|
日付 |
2025-10-24 |
|
日付タイプ |
Available |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_5794 |
|
資源タイプ |
conference paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| ID登録 |
|
|
ID登録 |
10.15027/0002041276 |
|
ID登録タイプ |
JaLC |
| 書誌情報 |
en : Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan
巻 20,
p. 97-100,
ページ数 4,
発行日 2025-10-24
|
| 見出し |
|
|
大見出し |
Toxins, Biosynthesis and Detection Methods |
|
言語 |
en |
21(pp.97-100).pdf (414 KB)
