| Item type |
デフォルト(1) |
| 公開日 |
2025-10-14 |
| タイトル |
|
|
タイトル |
Investigation of Limit of Detection and Quantification for digital PCR assays: the case study of Azadinium spinosum (Dinophyceae) in Irish waters |
|
言語 |
en |
| 作成者 |
Caputo, Nicolè
Clarke, Dave
Cucchi, Francesca
Kavanagh, Fiona
Mirimin, Luca
|
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Imai, Ichiro |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Matsushima, Ryoji |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nagai, Satoshi |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nishitani, Goh |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Sakamoto, Setsuko |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Suzuki, Toshiyuki |
|
|
言語 |
en |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Azadinium spinosum |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
azaspiracids |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
quantitative PCR |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
digital PCR |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
LOD |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
LOQ |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
monitoring |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Azaspiracids (AZAs) are a group of lipophilic toxins associated with Azaspiracid Poisoning (AZP) in humans following consumption of contaminated shellfish. AZA toxins are produced by some species of marine nano dinoflagellates from the cosmopolitan family Amphidomataceae. Ireland is one of the countries most affected globally by AZA events, where blooms of toxigenic Azadinium spinosum have been leading to prolonged closures of shellfish farms. DNA-based techniques such as quantitative PCR (qPCR) and digital PCR (dPCR) are extremely sensitive methods that have shown great potential for routine screening of environmental samples, with the former being successfully applied in the detection and relative quantification of some species of Amphidomataceae in Irish waters. In contrast, dPCR has been used to a lesser extent despite its ability to enable absolute quantification of low-copy targets and potential to mitigate enzymatic inhibitory effects. Thus, the present study tested the accuracy of two dPCR systems (Standard BioTools’ BiomarkHD and QIAGEN’s QIAcuity Four) using a TaqMan probe-based assay targeting the LSU-rDNA gene in samples of known target concentration. Limit of Detection (LOD) and Quantification (LOQ) thresholds were defined to infer transferability, repeatability, and reproducibility of the assay across the different systems. Both dPCR methods showed a positive correlation between and within platforms, and high sensitivity and accuracy in detecting and
quantify low-copy targets. These are promising results especially in view of implementing these platforms into National Monitoring Programmes for monitoring harmful phytoplankton. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
本文は、ISSHAのHP（ リンク：https://issha.org/publications-resources/conference-proceedings/ ）にて公開。 |
|
言語 |
ja |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This project (Grant-aid Agreement No. CS-21-007) is carried out with the support of the Marine Research Programme by the Irish Government. Mobility grants support: Paul C. Silva Student Grant funded by the International Phycological Society; Networking and Marine Research Communication Award funded by the Marine Institute under the Marine Research Programme with the support of the Irish Government. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Ichiro Imai, Ryoji Matsushima, Satoshi Nagai, Goh Nishitani, Setsuko Sakamoto, Toshiyuki Suzuki (Eds.). 2023. Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan.
International Society for the Study of Harmful Algae. 【69-72】 pp. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
International Society for the Study of Harmful Algae |
|
言語 |
en |
| 日付 |
|
|
日付 |
2025-10-24 |
|
日付タイプ |
Available |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_5794 |
|
資源タイプ |
conference paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| ID登録 |
|
|
ID登録 |
10.15027/0002041269 |
|
ID登録タイプ |
JaLC |
| 書誌情報 |
en : Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan
巻 20,
p. 69-72,
ページ数 4,
発行日 2025-10-24
|
| 見出し |
|
|
大見出し |
Monitoring and Mitigation |
|
言語 |
en |
14(pp.69-72).pdf (277 KB)
