| Item type |
デフォルト(1) |
| 公開日 |
2025-10-14 |
| タイトル |
|
|
タイトル |
Application of a quantitative molecular methods to characterize abundance and distribution of Alexandrium cysts for NOAA’s HAB Forecasting |
|
言語 |
en |
| 作成者 |
Greengrove, Cheryl
Kibler, Steve
Masura, Julie
Matweyou, Julie
Hart, Courtney
Vandersea, Mark
Harman, Tyler
|
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Imai, Ichiro |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Matsushima, Ryoji |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nagai, Satoshi |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Nishitani, Goh |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Sakamoto, Setsuko |
|
|
言語 |
en |
| 寄与者 |
|
|
寄与者タイプ |
Editor |
|
|
姓名 |
Suzuki, Toshiyuki |
|
|
言語 |
en |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Alexandrium catenella |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cysts |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
qPCR |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
forecast |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Gulf of Maine |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Puget Sound |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Alaska |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Alexandrium catenella is a paralytic shellfish poisoning-causing dinoflagellate that overwinters as a resting cyst and germinates into the water column in the spring-summer. Using winter cyst distribution, a Gulf of Maine forecast and an early warning system in Puget Sound, Washington were developed to mitigate human health risks and economic effects of shellfish closures. The current protocol for A. catenella cyst enumeration by fluorescent microscopy is time consuming and requires highly specific training for cyst identification. This MERHAB project, funded by NOAA, includes development of a new quantitative polymerase chain reaction (qPCR) methodology for A. catenella cysts that is being evaluated against the standard microscopy protocol with the goal of producing more rapid and accurate cyst abundance data. Comparison of microscopy- and qPCR-based cyst abundances shows a strong correlation, but quantitative estimates of cysts using the qPCR method tends to overestimate cyst numbers. Assay development showed the importance of using a qPCR standard curve based on cysts instead of a diluted amplicon, necessitating concentration of a large number of A. catenella cysts from the environment at the time of the survey. Furthermore, interannual and regional variability in rDNA gene copy number requires development of a new standard curve for each cyst survey. qPCR standard curves were constructed and compared across three regions using cysts from the Gulf of Maine, Puget Sound and Kodiak, Alaska. To assess temporal variability in rDNA gene copy number, seasonal comparison of qPCR standard curves was completed for Quartermaster Harbor, Puget Sound over one year. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
本文は、ISSHAのHP（ リンク：https://issha.org/publications-resources/conference-proceedings/ ）にて公開。 |
|
言語 |
ja |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This project is funded by NOAA National Centers for Coastal Ocean Science (NCCOS) MERHAB grant number NA19NOS4780188 |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Ichiro Imai, Ryoji Matsushima, Satoshi Nagai, Goh Nishitani, Setsuko Sakamoto, Toshiyuki Suzuki (Eds.). 2023. Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan.
International Society for the Study of Harmful Algae. 【41-44】 pp. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
International Society for the Study of Harmful Algae |
|
言語 |
en |
| 日付 |
|
|
日付 |
2025-10-24 |
|
日付タイプ |
Available |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_5794 |
|
資源タイプ |
conference paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| ID登録 |
|
|
ID登録 |
10.15027/0002041262 |
|
ID登録タイプ |
JaLC |
| 書誌情報 |
en : Proceedings of the 20th International Conference on Harmful Algae, Hiroshima, Japan
巻 20,
p. 41-44,
ページ数 4,
発行日 2025-10-24
|
| 見出し |
|
|
大見出し |
Prediction and Modeling |
|
言語 |
en |
07(pp.41-44).pdf (356 KB)
