{"created":"2025-03-02T09:44:26.953425+00:00","id":2032758,"links":{},"metadata":{"_buckets":{"deposit":"8c0fe934-d86e-4991-855e-6f3e31044035"},"_deposit":{"created_by":41,"id":"2032758","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2032758"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02032758","sets":["1727147343679:1730444459898:1730444464731"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"ペプチド作動性Na+チャネルの活性化とイオン透過性を制御する機能部位 <学位論文要旨>","subitem_title_language":"ja"},{"subitem_title":"Functional sites controlling the activation and ion permeation of a peptide-gated Na+ channel <Summaries of the Doctoral Theses>","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小谷, 侑","creatorNameLang":"ja"},{"creatorName":"Kodani, Yu","creatorNameLang":"en"}],"familyNames":[{"familyName":"小谷","familyNameLang":"ja"},{"familyName":"Kodani","familyNameLang":"en"}],"givenNames":[{"givenName":"侑","givenNameLang":"ja"},{"givenName":"Yu","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2012 by Graduate School of Integrated Arts and Sciences, Hiroshima University All rights reserved"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Peptides are ubiquitous signaling molecules in animals. Most of the peptide receptors identified so far are G protein-coupled receptors, but peptide-gated Na+ channels have also been cloned. This study investigated the structurefunction relationship of a peptide(FMRFamide)- gated Na+ channel (FaNaC). We focused on three polar residues (Y548, D552, D556) around the outer end of the second transmembrane domain of FaNaC. Site-directed mutagenesis and cysteine modification experiments uncovered that D552 is a critical determinant for various functional aspects of FaNaC, such as activation and desensitization kinetics, apparent FMRFamide sensitivity, channel block by FMRFamide, and current rectification. Double-mutant cycle analysis demonstrated that the interaction between D552 and Y548 regulates both activation and desensitization processes. Although point mutants in D556 were nonfunctional, a double mutant D552N/D556N was functional and showed markedly slow current kinetics. External Ca2+ inhibited the activation and desensitization of FaNaC. These Ca2+ actions were diminished by removal of the negative charge of D552, and notably, the Ca2+ action on desensitization was completely abolished in D552N/D556N, suggesting that D552 and D556 are involved in the Ca2+-binding site. In summary, Y548, D552, and D556 are important sites controlling the gating of FaNaC, and D552 also affects the ion permeation of the channel.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"広島大学大学院総合科学研究科"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"1881-7688","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12198647","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"51"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-12-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"53","bibliographicPageStart":"51","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"広島大学大学院総合科学研究科紀要. I, 人間科学研究"},{"bibliographic_title":"Bulletin of the Graduate School of Integrated Arts and Sciences, Hiroshima University. I, Studies in human sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"StudiesInHumanSciences_7_51.pdf","filesize":[{"value":"1.0 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2032758/files/StudiesInHumanSciences_7_51.pdf"},"version_id":"f986fc05-1d9b-4275-be90-cd060bf74af1"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"34622"},"item_title":"ペプチド作動性Na+チャネルの活性化とイオン透過性を制御する機能部位 <学位論文要旨>","item_type_id":"40003","owner":"41","path":["1730444464731"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2032758","relation_version_is_last":true,"title":["ペプチド作動性Na+チャネルの活性化とイオン透過性を制御する機能部位 <学位論文要旨>"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-03-02T09:44:35.035660+00:00"}