{"created":"2025-03-01T01:58:19.064451+00:00","id":2023885,"links":{},"metadata":{"_buckets":{"deposit":"d4ada737-3014-4fa2-9cdd-f3e504e20c05"},"_deposit":{"created_by":41,"id":"2023885","owner":"41","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2023885"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02023885","sets":["1727147343679:1730443524288:1730443527864"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"RT-PCRによるブリからのPhotobacterium damselaの検出","subitem_title_language":"ja"},{"subitem_title":"Detection of Photobacterium damsela from Yellowtail by RT-PCR","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松岡, 学","creatorNameLang":"ja"},{"creatorName":"Matsuoka, Satoru","creatorNameLang":"en"}],"familyNames":[{"familyName":"松岡","familyNameLang":"ja"},{"familyName":"Matsuoka","familyNameLang":"en"}],"givenNames":[{"givenName":"学","givenNameLang":"ja"},{"givenName":"Satoru","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"廣瀬, 恵子","creatorNameLang":"ja"},{"creatorName":"Hirose, Keiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"廣瀬","familyNameLang":"ja"},{"familyName":"Hirose","familyNameLang":"en"}],"givenNames":[{"givenName":"恵子","givenNameLang":"ja"},{"givenName":"Keiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"惣明, 睦枝","creatorNameLang":"ja"},{"creatorName":"Soumyo, Yoshie","creatorNameLang":"en"}],"familyNames":[{"familyName":"惣明","familyNameLang":"ja"},{"familyName":"Soumyo","familyNameLang":"en"}],"givenNames":[{"givenName":"睦枝","givenNameLang":"ja"},{"givenName":"Yoshie","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"西澤, 豊彦","creatorNameLang":"ja"},{"creatorName":"Nishizawa, Toyohiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"西澤","familyNameLang":"ja"},{"familyName":"Nishizawa","familyNameLang":"en"}],"givenNames":[{"givenName":"豊彦","givenNameLang":"ja"},{"givenName":"Toyohiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"室賀, 清邦","creatorNameLang":"ja"},{"creatorName":"Muroga, Kiyokuni","creatorNameLang":"en"}],"familyNames":[{"familyName":"室賀","familyNameLang":"ja"},{"familyName":"Muroga","familyNameLang":"en"}],"givenNames":[{"givenName":"清邦","givenNameLang":"ja"},{"givenName":"Kiyokuni","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Photobacterium damsela subsp. damsela","subitem_subject_scheme":"Other"},{"subitem_subject":"Photobacterium damsela subsp. piscicida","subitem_subject_scheme":"Other"},{"subitem_subject":"Pasteurella piscicida","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"rRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"carrier","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"魚類病原細菌 Photobacterium damsela subsp. damsela および Ph. damsela subsp. piscicida を検出するために、本菌の 16S rRNA における種特異的塩基配列の解析に基づきRT-PCR法を考案した。まず、魚介類病原菌を中心とした12種のビブリオ属細菌を用いて本RT-PCRの特異性を確認し、また検査魚の脾臓磨砕液をトリプトソーヤ・ブロスで前培養後RT-PCRに供することにより検出感度が高まることが分かった。この方法を用いることにより、外見的に健康な養殖ブリ Seriola quinqueradiata およびウマヅラハギ Thamnaconus modestus やマアジ Trachurus japonicus など数種の天然魚に Ph. damsela が存在することが明らかにされた。","subitem_description_language":"ja"},{"subitem_description":"An RT-PCR detection technique was developed for Pholobacterium damsela (=Photobacterium damsela subsp. damsela and Ph. damsela subsp. piscicida) from apparently healthy yellowtail (Seriola quinqueradiata) and other wild fishes, based on the analysis of a species-specific sequence in 16S rRNA of Ph. damsela. Specificity of the RT-PCR with K-F1 (sense primer) and R-GEN (antisense primer) was confirmed by using Ph. damsela subsp. damsela and Ph. damsela subsp. piscicida, 12 strains of fish—associated Vibrio species. Sensitivity of the RT-PCR was improved by combining a pre-culture of the sample (spleen homogenate) with tryptic soy broth (NaCl 2%). This RT-PCR revealed the presence of Ph. damsela from wild yellowtail, filefish (Thamnaconus modestus) and horse mackerel (Trachurus japonicas) caught in the sea as well as apparently healthy cultured yellowtail.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"広島大学生物生産学部"},{"subitem_publisher":"農林水産研究情報センター"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_1617186819068":{"attribute_name":"identifier_registration","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15027/24728","subitem_identifier_reg_type":"JaLC"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"1341-691X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN10409040","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"139"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"146","bibliographicPageStart":"139","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"生物生産学研究"},{"bibliographic_title":"Journal of the Faculty of Applied Biological Science, Hiroshima University"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"57-0603.pdf","filesize":[{"value":"1.2 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2023885/files/57-0603.pdf"},"version_id":"ef744538-df17-439a-88ae-a8a8fc33014a"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"24728"},"item_title":"RT-PCRによるブリからのPhotobacterium damselaの検出","item_type_id":"40003","owner":"41","path":["1730443527864"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2023885","relation_version_is_last":true,"title":["RT-PCRによるブリからのPhotobacterium damselaの検出"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-04T07:42:23.064419+00:00"}