| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Modulation of Ciliary Activity by Tumor Necrosis Factor-alpha in Cultured Sinus Epithelial Cells. Possible Roles of Nitric Oxide. |
|
言語 |
en |
| 作成者 |
Chen, Jiu Hong
Takeno, Sachio
Osada, Rika
Ueda, Toshiyuki
Yajin, Koji
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Tumor necrosis factor alpha |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Nitric oxide |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ciliary beat frequency |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Laser scanning confocal microscopy |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
The primary function of well-differentiated ciliated epithelium in the paranasal sinus is to eliminate harmful agents through the beating action of cilia. Respiratory epithelium also contributes to local inflammatory processes through the release of various proinflammatory cytokines. Recently, considerable attention has been focused on the intimate relationship between the cytokine-dependent regulation of the ciliary beat frequency (CBF) and intra-cellular production of nitric oxide (NO) in ciliated epithelial cells. The aims of this study are to examine the effect of tumor necrosis factor-alpha (TNF-α), one of the major proinflammatory cytokines, on the ciliary activity of human sinus epithelial cells and to assess the hypothesis that NO is involved in this regulatory mechanism. Human maxillary or ethmoidal sinus mucosa (n=23) were cultured by the explant-outgrowth method. CBF of the outgrowth ciliated cells was measured by the photoelectrical method before and after being treated with TNF-α (0.1, 1 and 10 ng/ml) or dexamethasone (10⁻⁶M and 10⁻⁷M). We also investigated the expression of nitric oxide synthase (NOS) isoforms, enzymes responsible for NO synthesis, by fluorescent immunohistochemistry. TNF-αincreased CBF at relatively low concentrations (0.1 and 1 ng/ml) and decreased CBF at a high concentration (10 ng/ml). Dexamethasone decreased CBF at a concentration of 10⁻⁶M. Fluorescent immunohistochemistry demonstrated that the expression of inducible NOS was augmented by TNF-αand attenuated by dexamethasone, whereas that of endothelial NOS remained unchanged. We conclude that human sinus epithelial cells potentially contribute to the inflammatory process by regulating their ciliary motility through an NO-dependent pathway. Proinflammatory cytokines and steroids are able to modulate this mechanism by the induction or inhibition of expression of different NOS isoforms. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
A part of this work was supported by Grants -in -Aid for Scientific Research (NO. 11671681-00 to Koji Yajin) from the Ministry of Education, Science, Sports and Culture, Japan, and the Fund for research (to Jiu Hong CHEN) from the Society for Promotion of International Otorhinolaryngology (SPIO). |
| 出版者 |
|
|
出版者 |
Hiroshima University Medical Press |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0018-2052 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00664312 |
| 開始ページ |
|
|
開始ページ |
49 |
| 書誌情報 |
Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences
巻 49,
号 1,
p. 49-55,
発行日 2000-03
|
| 旧ID |
37701 |
HiroshimaJMedSci_49_49.pdf (1.1 MB)
