{"created":"2025-02-23T06:33:59.153355+00:00","id":2013461,"links":{},"metadata":{"_buckets":{"deposit":"79ca64f5-7a9e-480e-8aec-932d874394b3"},"_deposit":{"created_by":41,"id":"2013461","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2013461"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02013461","sets":["1727147343679:1730442224523:1730442296094"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Transcriptionally Targeted In Vivo Gene Therapy for Carcinoembrionic Antigen-Producing Adenocarcinoma","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Konishi, Futoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Konishi","familyNameLang":"en"}],"givenNames":[{"givenName":"Futoshi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Maeda, Hiroyuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Maeda","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiroyuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamanishi, Yuji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamanishi","familyNameLang":"en"}],"givenNames":[{"givenName":"Yuji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hiyama, Keiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hiyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Keiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishioka, Shinichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ishioka","familyNameLang":"en"}],"givenNames":[{"givenName":"Shinichi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamakido, Michio","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamakido","familyNameLang":"en"}],"givenNames":[{"givenName":"Michio","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Adenovirus-vector","subitem_subject_scheme":"Other"},{"subitem_subject":"HSV-TK","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin-6","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell-type-specific gene therapy","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Inoperable adenocarcinoma in colon or lung shows resistance to conventional anti-cancer therapy. For these cancers, the feasibility of transcriptionally targeted killing of carcinoembryonic antigen (CEA)-producing adenocarcinoma cells was investigated.　　    Adenovirus vectors carrying a CEA promoter to express E. coli lacZ (AdCEALacZ) or herpes simplex thymidine kinase (AdCEATK) were made and their in vitro and in vivo tumoricidal effects on CEA-producing or non-producing colon and lung cancer cells were evaluated.　　    In vitro infection with AdCEALacZ showed significantly higher CEA promoter-driven lacZ expression in CEA-producing adenocarcinoma cells including VMRC-LCD and LoVo than in CEA-non-producing cells. AdCEATK-infected LoVo showed higher sensitivity to ganciclovir than control vector-infected LoVo or AdCEATK-infected HeLa both in vitro and in subcutaneously implanted tumors of nude mice. Moreover, total tumor elimination in vivo was achieved by either pre-infection of as few as 30% of cells comprising tumors or by direct in vivo injection of AdCEATK to pre-established LoVo tumors. In addition, CEA promoter-driven lacZ expression in Lo Vo cells was enhanced by the addition ofinterleukin-6 (IL-6) in vitro.　　    These results provide a rationale for CEA-promoter-driven, adenovirus-mediated gene therapy for CEA-producing adenocarcinomas in colon and lung with reduced toxicity to normal cells.","subitem_description_language":"ja"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University Medical Press"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"79"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"89","bibliographicPageStart":"79","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_48_79.pdf","filesize":[{"value":"1.3 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2013461/files/HiroshimaJMedSci_48_79.pdf"},"version_id":"72d02d05-e4d1-465a-96a3-d3941206a4ec"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"37734"},"item_title":"Transcriptionally Targeted In Vivo Gene Therapy for Carcinoembrionic Antigen-Producing Adenocarcinoma","item_type_id":"40003","owner":"41","path":["1730442296094"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2013461","relation_version_is_last":true,"title":["Transcriptionally Targeted In Vivo Gene Therapy for Carcinoembrionic Antigen-Producing Adenocarcinoma"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T03:12:04.608701+00:00"}