{"created":"2025-02-23T06:32:04.547733+00:00","id":2013395,"links":{},"metadata":{"_buckets":{"deposit":"d4eb172a-90e6-41aa-a779-d23b706bd79a"},"_deposit":{"created_by":41,"id":"2013395","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2013395"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02013395","sets":["1727147343679:1730442224523:1730442305026"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"HLA-DR8 Subtyping by Polymerase Chain Reaction (PCR) - DNA Conformation Polymorphism (DCP) Analysis : a simple and practical genotyping method","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fukuda, Yasuhiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Fukuda","familyNameLang":"en"}],"givenNames":[{"givenName":"Yasuhiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kimura, Akinori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kimura","familyNameLang":"en"}],"givenNames":[{"givenName":"Akinori","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hoshino, Shuji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hoshino","familyNameLang":"en"}],"givenNames":[{"givenName":"Shuji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shintaku, Sadanori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Shintaku","familyNameLang":"en"}],"givenNames":[{"givenName":"Sadanori","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sakaguchi, Takemasa ","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sakaguchi","familyNameLang":"en"}],"givenNames":[{"givenName":"Takemasa ","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Asahara, Toshimasa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Asahara","familyNameLang":"en"}],"givenNames":[{"givenName":"Toshimasa","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sakaki, Miyoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sakaki","familyNameLang":"en"}],"givenNames":[{"givenName":"Miyoko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sumimoto, Ryo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sumimoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Ryo","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tashiro, Hirotaka","creatorNameLang":"en"}],"familyNames":[{"familyName":"Tashiro","familyNameLang":"en"}],"givenNames":[{"givenName":"Hirotaka","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Furukawa, Masahiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Furukawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Masahiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohdan, Hideki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ohdan","familyNameLang":"en"}],"givenNames":[{"givenName":"Hideki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yoshida, Tetsuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yoshida","familyNameLang":"en"}],"givenNames":[{"givenName":"Tetsuya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Dohi, Kiyohiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Dohi","familyNameLang":"en"}],"givenNames":[{"givenName":"Kiyohiko","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"PCR (polymerase chain reaction)","subitem_subject_scheme":"Other"},{"subitem_subject":"Subcloning, HLA (human leukocyte antigen)","subitem_subject_scheme":"Other"},{"subitem_subject":"SSO (sequence-specific oligonucleotide) typing","subitem_subject_scheme":"Other"},{"subitem_subject":"Sanger's dideoxy sequencing method","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Two hundred Japanese panels were serologically typed for human leukocyte antigen (HLA) - DR to assign 65 HLA-DRS haplotypes, which were then subdivided into two genotypes, i.e., DRB1 *0802 and DRB1 *0803, by a polymerase chain reaction (PCR) - based, simple, and practical method. The panels possessing DR8 specificity were firstly subjected to PCR with a couple of primers specifically to amplify their DR52 associated group - DRB1 genes. PCR products were then denatured in the presence of formamide, electrophoresed in a non-denaturing polyacrylamide gel, and visualized by silver staining. The same DRB1 products of these samples were also mixed with the DRB1 *1302, and simultaneously analyzed by the same procedure. Electrophoretic mobilities of the samples were compared with those of the typing standards to genotype their DRS-DRB1 alleles by using the characteristic polymorphism in the single-stranded DNAs and the heteroduplexes. This method, designated PCR - DNA conformation polymorphism (DCP) analysis, allowed for genotyping of the DR8-DRB1 alleles without using sequence - specific oligonucleotide probes (SSOP) or restriction endonucleases. The entire process after PCR was completed within a few hours. The tested panels were also genotyped for DRB1 gene by the PCR-SSOP method for comparison with results obtained by the PCR-DCP method. Satisfactory coincidence was achieved and it represented how accurately the new system genotyped DRB1 *0802 and DRB1 *0803. PCR-DCP analysis was thus shown to be practical and useful for subtyping of serologically defined DR8 specificities.","subitem_description_language":"en"},{"subitem_description":"This work was supported in part by grants-in-aid from the Ministry of Education, Culture and Science, Japan, and research grants from the Ministry of Health and Welfare, Japan.","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University Medical Press"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"85"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"92","bibliographicPageStart":"85","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_45_85.pdf","filesize":[{"value":"946.4 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2013395/files/HiroshimaJMedSci_45_85.pdf"},"version_id":"a963af9e-291e-45a5-a8b6-e0f927c272eb"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"37845"},"item_title":"HLA-DR8 Subtyping by Polymerase Chain Reaction (PCR) - DNA Conformation Polymorphism (DCP) Analysis : a simple and practical genotyping method","item_type_id":"40003","owner":"41","path":["1730442305026"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2013395","relation_version_is_last":true,"title":["HLA-DR8 Subtyping by Polymerase Chain Reaction (PCR) - DNA Conformation Polymorphism (DCP) Analysis : a simple and practical genotyping method"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T03:08:32.376272+00:00"}