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Inhibition of 3H-TdR uptake for Human Gastric Carcinoma Cells by Epidermal Growth Factor Specific Antibody
https://hiroshima.repo.nii.ac.jp/records/2013274
https://hiroshima.repo.nii.ac.jp/records/2013274107b5bc1-c5f5-46f4-8d4b-cec47b43de56
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HiroshimaJMedSci_40_15.pdf (717.3 KB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||
| タイトル | ||||||||
| タイトル | Inhibition of 3H-TdR uptake for Human Gastric Carcinoma Cells by Epidermal Growth Factor Specific Antibody | |||||||
| 言語 | en | |||||||
| 作成者 |
Kyo, Eikai
× Kyo, Eikai
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | EGF | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | TGFα | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Autocrine growth factor | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Anti-EGF monoclonal antibody | |||||||
| 主題 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 490 | |||||||
| 内容記述 | ||||||||
| 内容記述 | This study examined whether human gastric carcinoma cells produced EGF for self-stimulation, using TMK-1 and MKN-28 cells which express mRNA for EGF and EGF receptor. EGF protein production was confirmed by ELISA and flow cytometric analysis. The lysates of these cells replaced EGF-induced biological activity to EGF receptor bearing KB cells but the culture supernatants did not. Furthermore, EGF-radioreceptor assay revealed non-secretion of EGF product. EGF specific monoclonal antibody neutralized exogenous EGF-induced 3H-TdR uptake, and inhibited spontaneous 3H-TdR uptake of TMK-1 and MKN-28 cells. These results suggest that EGF specific antibody binds to EGF protein at the cell surface, leading to the inhibition of 3HTdR uptake, and that EGF produced by tumor cells may act as an autocrine growth factor in human gastric carcinoma cells. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | This study was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy for Cancer Control, Japan. | |||||||
| 出版者 | ||||||||
| 出版者 | Hiroshima University Medical Press | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 1864762 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0018-2052 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00664312 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 15 | |||||||
| 書誌情報 |
Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 巻 40, 号 1, p. 15-21, 発行日 1991-03 |
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| 旧ID | 38007 | |||||||
