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Multidirectionality of Anti-Platelet Antibodies in Idiopathic Thrombocytopenic Purpura
https://hiroshima.repo.nii.ac.jp/records/2013221
https://hiroshima.repo.nii.ac.jp/records/2013221ed1b5303-77c6-4808-b1fc-f0b2ab96a920
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HiroshimaJMedSci_38_75.pdf (881.9 KB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||
| タイトル | ||||||||
| タイトル | Multidirectionality of Anti-Platelet Antibodies in Idiopathic Thrombocytopenic Purpura | |||||||
| 言語 | en | |||||||
| 作成者 |
Oda, Kenji
× Oda, Kenji
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | ITP | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | PA-IgG | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Platelet antigen | |||||||
| 主題 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 490 | |||||||
| 内容記述 | ||||||||
| 内容記述 | Chronic idiopathic thrombocytopenic purpura (chronic ITP) is an autoimmune disorder characterized by bleeding tendency and the presence of auto-antibody to platelets. Quantitative analysis of the level of anti-platelet antibodies and platelet associated antibodies is now possible. Furthermore, recent immunological techniques have enabled us to detect target platelet antigens in chronic ITP. In this study, (1) anti-platelet antibodies, contained in serum, were examined by an indirect platelet suspension immunofluorescence test (indirect PSIFT) for IgG+A+M, and platelet bind-able IgG (PB-IgG) was measured quantitatively by enzyme linked immunosolvent assay (ELISA). Indirect PSIFT was positive in 4.2%, and PB-IgG was elevated in 12.5% of ITP patients. (2) Platelet associated antibodies were detected by direct PSIFT and platelet associated IgG (PA-IgG) were assayed by solid phase competitive ELISA. Direct PSIFT was positive in 34.1 % and PA-IgG was elevated in 37.8% (3) IgG antibodies against platelet lipid compounds (cardiolipin and sulfatides) were also measured by ELISA. Anti-cardiolipin antibody was elevated in 56.3% and anti-sulfatides antibody was elevated in 31.3%. Anti-cardiolipin antibodies correlated well with anti-sulfatides antibody, and also correlated with PB-IgG. (4) Target platelet antigens were detected by immunoprecipitation and Western blotting. Immunoprecipitation showed multiple antigenic bindings in one out of 19 ITP patients with positive serum anti-platelet antibody. On Western blotting analysis, multiple antigenic binding sites were detected in six out of 48 patients. The data in this study are consistent with the idea that auto-antibodies in ITP are not directed towards a single protein, but to multiple platelet antigens which are included in proteins and lipid compounds. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | Hiroshima University Medical Press | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 2793519 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0018-2052 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00664312 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 75 | |||||||
| 書誌情報 |
Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 巻 38, 号 2, p. 75-86, 発行日 1989-06 |
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| 旧ID | 38109 | |||||||
