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Tyrosine Tubulin Kinases in Particulate Fractions from Rat Cerebral Cortex
https://hiroshima.repo.nii.ac.jp/records/2013176
https://hiroshima.repo.nii.ac.jp/records/2013176fa8ab092-517d-4b14-be8f-0974b944800c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HiroshimaJMedSci_36_395.pdf (5.5 MB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||
| タイトル | ||||||||
| タイトル | Tyrosine Tubulin Kinases in Particulate Fractions from Rat Cerebral Cortex | |||||||
| 言語 | en | |||||||
| 作成者 |
TSUKAMOTO, Haruhisa
× TSUKAMOTO, Haruhisa
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Cerebral cortex | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Tyrosine protein kinase | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | pp60^c-src | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Tubulin | |||||||
| 主題 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 490 | |||||||
| 内容記述 | ||||||||
| 内容記述 | Tyrosine tubulin kinase activity in the particulate fractions from rat cerebral cortex was quantitatively solubilized and separated into two active peaks (kinase I and kinase II) by Sephacryl S-300 gel-filtration in the presence of 0.2% Nonidet P-40. Kinases I and II were each resolved into 5 active peaks (I-1→5 and II-1→5) by casein-Sepharose column chromatography. The molecular weights of these kinases were estimated from the s20,w values to be 59,000-65,000. Tyrosine-glutamate (1:4) copolymers were also substrates for the enzymes. About 60% of the copolymers kinase activity in I-3, I-4, II-3 and II-4 were immunoprecipitable with saturating amount of monoclonal antibody (MAb 327) that recognizes pp60^c-src. The Km values of II-3 and II-4 for tubulin were nearly 10 times higher than those of I-3 and I-4. However, the Km values of the four kinases for the copolymers were not so significantly different. In comparison with the II-3 and II-4 kinase fractions, I-3 and I-4 fractions showed 2 and 10 times higher activity ratios with tubulin and the IgG heavy chain of MAb 327 relative to the copolymers kinase activity. Incubation of the immunocomplexes with ATP at 0°C for 10 min resulted in the autophosphorylation of a 60kDa protein in I-3 and I-4 but not in II-3 and II-4. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | Hiroshima University Medical Press | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 3129380 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0018-2052 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00664312 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 395 | |||||||
| 書誌情報 |
Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 巻 36, 号 4, p. 395-406, 発行日 1987-12 |
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| 旧ID | 50007 | |||||||
