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Ultracytochemical Demonstration of Several Phosphatases in the Human Corpus Luteum of Early Pregnancy by a Cerium-Based Method in Combination with a Microslicer
https://hiroshima.repo.nii.ac.jp/records/2013170
https://hiroshima.repo.nii.ac.jp/records/2013170f4d2c6ed-9010-4eeb-8410-0d4535113cb1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HiroshimaJMedSci_36_347.pdf (10.2 MB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||
| タイトル | ||||||||
| タイトル | Ultracytochemical Demonstration of Several Phosphatases in the Human Corpus Luteum of Early Pregnancy by a Cerium-Based Method in Combination with a Microslicer | |||||||
| 言語 | en | |||||||
| 作成者 |
MYOGA, Hiroshi
× MYOGA, Hiroshi
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Phosphatase | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Corpus luteum | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Cerium | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Microslicer | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Ultracytochemistry | |||||||
| 主題 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 490 | |||||||
| 内容記述 | ||||||||
| 内容記述 | Acid phospatase (AcPase), alkaline phosphatase (AlPase), 5'-nucleotidase (AMPase), Mg-activated adenosine triphospatase (ATPase), thiamine pyrophospatase (TPPase) and glucose-6-phospatase (G6Pase) were investigated in the human corpus luteum of early pregnancy using a cerium-based method in combination with a microslicer. Ultrastructurally, cerium phosphate reaction product appears as a very fine electron dense precipitate. The AcPase reaction products were exclusively localized in the lysosomes and in two cisternae of the Golgi lamellae on the trans-side. The AlPase and AMPase reaction products were observed in the plasma membrane. The Mg-activated ATPase reaction products were found in the matrix of the mitochondria as well as in the plasma membrane. The TPPase reaction products were present in two cisternae of the Golgi lamellae on the trans-side. The G6Pase reaction products were found in the rough endoplasmic reticulum. This technique can be applied routinely for the demonstration of phosphatase activity because of its reproducibility and specificity when cerium is used as the capturing agent, and yields better results than the lead-based method. In the luteal cells, these several phosphatases might serve to maintain a constant metabolic activity in the early pregnancy. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | Hiroshima University Medical Press | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 2834307 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0018-2052 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00664312 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 347 | |||||||
| 書誌情報 |
Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 巻 36, 号 4, p. 347-355, 発行日 1987-12 |
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| 旧ID | 50001 | |||||||
