{"created":"2025-02-23T06:24:13.182290+00:00","id":2013124,"links":{},"metadata":{"_buckets":{"deposit":"6da1e783-9411-43ba-985c-97940a660e9b"},"_deposit":{"created_by":41,"id":"2013124","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2013124"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02013124","sets":["1727147343679:1730442224523:1730442331848"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Direct Inhibitory Effects of 17β-Estradiol on hCG-Stimulated cAMP and Testosterone Responses of Canine Testis","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YASUKAWA, Akihiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"YASUKAWA","familyNameLang":"en"}],"givenNames":[{"givenName":"Akihiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"NIHIRA, Hiromi","creatorNameLang":"en"}],"familyNames":[{"familyName":"NIHIRA","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiromi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Estrogen","subitem_subject_scheme":"Other"},{"subitem_subject":"Prostatic cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"Androgen","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The direct effects of 17β-estradiol (E2) on testicular function were investigated using in situ perfusion of canine testis. During perfusion of E2 (10^-8-10^-4M), we measured the concentrations of cAMP, testosterone (T), androstenedione and 5α-dihydrotestosterone (DHT) in the spermatic venous effluent after hCG (500 IU) injection into the spermatic artery.  The cAMP concentration in the spermatic venous effluent was 122 ± 3. 7 (SE) pmoles/ml, increasing after hCG to a peak level of 259.1 ± 33.0 (SE) pmoles/ml at 45 min and decreasing thereafter. T increased gradually from the pretreated level of 12.2 ± 4.4 (SE) mg/ml to 90.9 ± 30.9 (SE) ng/ml at 90 min after hCG injection. Androstenedione production was also stimulated by hCG. Although the pattern of changes of DHT in the venous effluent was similar to that of T, a statistical significant increase was not observed after hCG injection. Continuous infusion with E2 at the concentrations of 10^-8-^-4 M decreased hCG-induced cAMP increase in a dose-dependent fashion. The peak level of cAMP after hCG was shown to be increasingly depleted (50 and 80%) by the increasing doses of 10^-6 and 10^-4 M of exogenous E2. The responses of T and androstenedione were also suppressed by E2 and at 10^-4 M of E2 no increase was observed, although the response of cAMP to hCG remained. These findings indicate that E2 can directly suppress the hCG-stimulated steroidogenesis and cAMP production in in situ canine testis.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University School of Medicine"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"7"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"12","bibliographicPageStart":"7","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"3034834","subitem_relation_type_select":"PMID"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_36_7.pdf","filesize":[{"value":"738.6 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2013124/files/HiroshimaJMedSci_36_7.pdf"},"version_id":"69c64dcb-e0bd-47b9-9e70-8e0ae95ea525"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"49955"},"item_title":"Direct Inhibitory Effects of 17β-Estradiol on hCG-Stimulated cAMP and Testosterone Responses of Canine Testis","item_type_id":"40003","owner":"41","path":["1730442331848"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2013124","relation_version_is_last":true,"title":["Direct Inhibitory Effects of 17β-Estradiol on hCG-Stimulated cAMP and Testosterone Responses of Canine Testis"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T02:57:26.712798+00:00"}