| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Preservation of Actin Immunoreactivity in Paraffin Section by Tannic Acid Fixation on Chick Embryonic Heart: Comparison with Phalloidin Staining on Frozen Section |
|
言語 |
en |
| 作成者 |
SUMIDA, Hiroshi
AKIMOTO, Naotaka
LEE, Juin Yi
SATOW, Yukio
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Actin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Tannic acid |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Phalloidin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Chick embryonic heart |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Degree of binding reactivity to actin in variously fixed tissues was histochemically examined by using phalloidin and antiactin staining on paraffin or frozen sections of 5-day chick embryonic heart. Fluorescein-labeled phalloidin staining on frozen section of the specimens fixed with 4% formaldehyde showed intense staining of actin in the myocardium and the aorticopulmonary (AP) septum. However, phalloidin did not react to actin on paraffin sections of the specimens fixed with 4% formaldehyde, nor fixed with 4% formaldehyde and 1% tannic acid (TA). FITC-labeled antiactin staining on paraffin sections of the specimens fixed with 4% formaldehyde and 1% TA showed comparable fluorescent intensity with that of phalloidin staining on frozen sections. However antiactin staining on paraffin sections of the specimens fixed with 4% formaldehyde only showed reduction of fluorescent intensity around the Ap septum. Both phalloidin and antiactin react non-specifically to the tissues fixed with solutions including 1% glutaraldehyde. Since paraffin sections showed much better tissue preservation than frozen sections, we suggest that paraffin embedding with TA fixation is very useful for actin histochemistry using antiactin. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Hiroshima University School of Medicine |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
3553094 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0018-2052 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00664312 |
| 開始ページ |
|
|
開始ページ |
347 |
| 書誌情報 |
Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences
巻 35,
号 4,
p. 347-353,
発行日 1986-12
|
| 旧ID |
49942 |
HiroshimaJMedSci_35_347.pdf (6.5 MB)
