{"created":"2025-02-23T06:19:15.659773+00:00","id":2012950,"links":{},"metadata":{"_buckets":{"deposit":"71f2e5df-8ca9-4cab-aec3-4fe88111ece4"},"_deposit":{"created_by":41,"id":"2012950","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2012950"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02012950","sets":["1727147343679:1730442224523:1730442339632"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Fluorometric Measurement of Neuraminidase Activity of Influenza Viruses","subitem_title_language":"en"},{"subitem_title":"螢光比色法によるインフルエンザウイルスノイラミニダーゼ活性の測定","subitem_title_language":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kiyotani, Katsuhiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kiyotani","familyNameLang":"en"}],"givenNames":[{"givenName":"Katsuhiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takei, Naomi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Takei","familyNameLang":"en"}],"givenNames":[{"givenName":"Naomi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsuo, Yoshiyasu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Matsuo","familyNameLang":"en"}],"givenNames":[{"givenName":"Yoshiyasu","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"4-Methylumbelliferyl-N-Ac-α-D-neuraminide","subitem_subject_scheme":"Other"},{"subitem_subject":"Neuraminidase","subitem_subject_scheme":"Other"},{"subitem_subject":"Influenza virus","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"A rapid and simple assay method for determining neuraminidase activity was investigated　fluorometrically with 4-methylumbelliferyl-N-Ac-α-D-neuraminide as a substrate.    The time required for performance was less than 3 hr and the activity could be　simply measured with a fluorescent spectrophotometer.    Neuraminidase activity in culture supernatant of MDCK cell infected with a small　quantity of influenza A/Aichi/2/68 virus was demonstrated by the fluorometric assay　24 hr after infection, when no viral cytopathogenic effect could be observed and no　viral multiplication could be demonstrated by the hemagglutination test and the colorimetric　neuraminidase assay method using fetuin as a substrate.    The result was that the minimum number of virion detectable by the fluorometric　neuraminidase assay method was approximately 10^3 order.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University School of Medicine"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"287"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1984-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"292","bibliographicPageStart":"287","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"6480393","subitem_relation_type_select":"PMID"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_33_287.pdf","filesize":[{"value":"382.8 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2012950/files/HiroshimaJMedSci_33_287.pdf"},"version_id":"a8565f49-b92e-4b09-b75c-c944199485ee"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"38215"},"item_title":"Fluorometric Measurement of Neuraminidase Activity of Influenza Viruses","item_type_id":"40003","owner":"41","path":["1730442339632"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2012950","relation_version_is_last":true,"title":["Fluorometric Measurement of Neuraminidase Activity of Influenza Viruses"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T02:53:19.824413+00:00"}