{"created":"2025-02-23T06:19:13.788725+00:00","id":2012949,"links":{},"metadata":{"_buckets":{"deposit":"2076122a-fcac-4e69-b4fb-6064e8887235"},"_deposit":{"created_by":41,"id":"2012949","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2012949"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02012949","sets":["1727147343679:1730442224523:1730442339632"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Firefly Luciferase Assay for Adenosine Triphosphate in Cultured Cells","subitem_title_language":"en"},{"subitem_title":"ルシフェラーゼ試薬を用いる培養細胞の ATP 測定法","subitem_title_language":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kiyotani, Katsuhiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kiyotani","familyNameLang":"en"}],"givenNames":[{"givenName":"Katsuhiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kanamoto, Yasuo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kanamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Yasuo","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsuo, Yoshiyasu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Matsuo","familyNameLang":"en"}],"givenNames":[{"givenName":"Yoshiyasu","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Firefly luciferase","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP","subitem_subject_scheme":"Other"},{"subitem_subject":"HeLa 229 cells","subitem_subject_scheme":"Other"},{"subitem_subject":"McCoy cells","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"A method for determining intracellular content of adenosine triphosphate (ATP) in the cultured cells was investigated with a firefly luciferase reagent, and a micro assay method was established. The method minimized the size of both samples and luciferase reagent employed.     The per cell amount of intracellular ATP of HeLa 229 and McCoy cells was estimated to be 7.20×10-11 M arid 8.74×10-11 M, respectively. The method could be applied to a direct measurement of the ATP content in cultured cells grown in each well of a 96-well micro tissue culture plate.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University School of Medicine"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"279"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1984-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"285","bibliographicPageStart":"279","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"6480392","subitem_relation_type_select":"PMID"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_33_279.pdf","filesize":[{"value":"388.7 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2012949/files/HiroshimaJMedSci_33_279.pdf"},"version_id":"d68ebe49-42cc-4a93-8e73-5bbf3db79440"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"38214"},"item_title":"Firefly Luciferase Assay for Adenosine Triphosphate in Cultured Cells","item_type_id":"40003","owner":"41","path":["1730442339632"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2012949","relation_version_is_last":true,"title":["Firefly Luciferase Assay for Adenosine Triphosphate in Cultured Cells"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T02:53:20.591092+00:00"}