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アイテム
ヒト頼粒膜細胞における分化とステロイド分泌能に関する電子顕微鏡的研究
https://hiroshima.repo.nii.ac.jp/records/2012873
https://hiroshima.repo.nii.ac.jp/records/201287346ad9279-04c3-4c44-b596-42d10690a684
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HiroshimaJMedSci_32_247.pdf (3.0 MB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Electron Microscopy of Cytodifferentiation and its Subcellular Steroidogenic Sites in the Granulosa Cell of the Human Ovary | |||||||||||
| 言語 | en | |||||||||||
| タイトル | ||||||||||||
| タイトル | ヒト頼粒膜細胞における分化とステロイド分泌能に関する電子顕微鏡的研究 | |||||||||||
| 言語 | ja | |||||||||||
| 作成者 |
Nagai, Nobutaka
× Nagai, Nobutaka
× Hiura, Masamichi
× Fujiwara, Atsushi
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| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Electron microscopy | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Granulosa cell | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Cytodifferentiation | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Steroidogenesis | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Human ovary | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | NDC | |||||||||||
| 主題 | 490 | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | The cytodifferentiation and subcellular steroidogenic sites in the granulosa cell of the developing follicle and in vitro effect of estradiol-17β (E2) on the granulosa cell of the preovulatory follicle in the human ovary were investigated using the electron microscopic cytochemistry. The follicular cell in the primordial follicle showed an elongated nucleus, rough endoplasmic reticulum, Golgi apparatus, rod-shaped mitochondria with lamellar cristae, free ribosomes and a few lipid droplets. In the secondary follicle, the granulosa cell derived from the follicular cell had a round nucleus, rough endoplasmic reticulum, lipid droplets, Golgi apparatus, microfilament, mitochondria with lamellar or tubular cristae and a small amount of smooth endoplasmic reticulum. Especially, the granulosa cell in the preovulatory follicle considered to be a transitional form to the steroid-secreting cell was characteristic of rough endoplasmic reticulum, lipid droplets, mitochondria with lamellar or tubular cristae and moderately well developed smooth endoplasmic reticulum. On the other hand, the granulosa cell in the postovulatory follicle showing a typical steroid-secreting activity had abundant lipid droplets, round mitochondrial with tubular or vesicular cristae, well developed smooth endoplasmic reticulum and lysosomes. The hallmarks of the cytodifferentiation of the granulosa cell were i) an appearance of lipid droplets, ii) a structural change of mitochondrial cristae from lamellar to tubular configuration and iii) an appearance and development of smooth endoplasmic reticulum. Reaction products for 3β-hydroxysteroid dehydrogenase (3β-HSD) activity were localized on tubular or lamellar cristae and inner membrane of the mitochondria, and on the membrane of smooth endoplasmic reticulum in the granulosa cell of the preovulatory as well as of the postovulatory follicle. The granulosa cell of the preovulatory follicle incubated in the medium containing E2 showed a structural change of mitochondrial cristae from lamellar to tubular configuration, and an appearance and development of smooth endoplasmic reticulum. From these facts, it is suggested that the granulosa cell in the preovulatory follicle has already a steroid-secreting activity and luteinizes abruptly, and E2 contained in the culture medium could stimulate the functional differentiation of the granulosa cell in the prevulatory follicle affected with gonadotrophin. | |||||||||||
| 言語 | en | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Hiroshima University School of Medicine | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| 関連情報 | ||||||||||||
| 識別子タイプ | PMID | |||||||||||
| 関連識別子 | 6643103 | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0018-2052 | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA00664312 | |||||||||||
| 開始ページ | ||||||||||||
| 開始ページ | 247 | |||||||||||
| 書誌情報 |
Hiroshima Journal of Medical Sciences Hiroshima Journal of Medical Sciences 巻 32, 号 3, p. 247-256, 発行日 1983-09 |
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| 旧ID | 38314 | |||||||||||
