{"created":"2025-02-23T06:16:50.838493+00:00","id":2012865,"links":{},"metadata":{"_buckets":{"deposit":"076d405e-bc6a-4d8f-8341-6f03dcac3f72"},"_deposit":{"created_by":41,"id":"2012865","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2012865"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02012865","sets":["1727147343679:1730442224523:1730442343133"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Isoenzyme Profiles of α-Mannosidase and β-Galactosidase in Leukemic Cells","subitem_title_language":"en"},{"subitem_title":"白血病細胞の α-マンノシダーゼと β-ガラクトシダーゼのアイソザイムパターン","subitem_title_language":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanaka, Takeo","creatorNameLang":"en"}],"familyNames":[{"familyName":"Tanaka","familyNameLang":"en"}],"givenNames":[{"givenName":"Takeo","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kobayashi, Masao","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kobayashi","familyNameLang":"en"}],"givenNames":[{"givenName":"Masao","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Usui, Tomofusa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Usui","familyNameLang":"en"}],"givenNames":[{"givenName":"Tomofusa","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Isoenzyme Profiles","subitem_subject_scheme":"Other"},{"subitem_subject":"α-mannosidase","subitem_subject_scheme":"Other"},{"subitem_subject":"β-galactosidase","subitem_subject_scheme":"Other"},{"subitem_subject":"Leukemia","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The isoenzyme profiles of α-mannosidase and β-galactosidase in leukemic cells were examined with DEAE-Sephadex chromatography. A clear difference of these constituent isoenzymes was found between normal lymphocytes and granulocytes. In leukemic cells, the components of a-mannosidase eluting early (fraction I to III) from the chromatography were specific for myelogenous leukemia. No case of acute non-T/ non-B lymphocytic leukemia (ALL) or T-cell leukemia demonstrated these components. Leukemic cells except for T-cell leukemia also demonstrated significant increase of an α-mannosidase component which eluted with 0.2 M of KCl (fraction VI).     There was no type specific alteration of β-galactosidase isoenzymes among examined leukemias. ALL and T-cell leukemia had isoenzymes of β-galactosidase similar to those of normal lymphocytes. However, an increase of an β-galactosidase component eluted with 0.3M of KCl (fraction VII) was found in acute myelocytic leukemia and chronic myelo (mono) cytic leukemia.    These enzyme analysis may be useful for investigating intrinsic abnormalities in leukemic cells.","subitem_description_language":"en"},{"subitem_description":"This work was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Hiroshima University School of Medicine"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0018-2052","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00664312","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"207"}]},"item_1617187056579":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1983-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"212","bibliographicPageStart":"207","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Hiroshima Journal of Medical Sciences"},{"bibliographic_title":"Hiroshima Journal of Medical Sciences"}]}]},"item_1617258105262":{"attribute_name":"item_1617258105262","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"6413460","subitem_relation_type_select":"PMID"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"HiroshimaJMedSci_32_207.pdf","filesize":[{"value":"411.1 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2012865/files/HiroshimaJMedSci_32_207.pdf"},"version_id":"7f6f8334-2297-4caa-b963-49872707ce0a"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"38341"},"item_title":"Isoenzyme Profiles of α-Mannosidase and β-Galactosidase in Leukemic Cells","item_type_id":"40003","owner":"41","path":["1730442343133"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2012865","relation_version_is_last":true,"title":["Isoenzyme Profiles of α-Mannosidase and β-Galactosidase in Leukemic Cells"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-06-02T02:51:18.020128+00:00"}