| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
An Improved Method for Determination of Serum Adenosine Deaminase Activity and its Clinical Applications |
|
言語 |
en |
| タイトル |
|
|
タイトル |
血清アデノシンデアミナーゼ活性の測定法の改良とその臨床的応用 |
|
言語 |
ja |
| 作成者 |
Shiosaka, Takahiko
Ohminami, Hiroji
Kobayashi, Yuzuru
Okuda, Hiromichi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Adenosine deaminase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Serum |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
An improved method was devised for the estimation of serum adenosine deaminase activity. The principle of the method consists in measurement of a liberated ammonia by the action of the deaminase with a direct colorimetric determination of ammonia concentration [Okuda et al.]. The technique is relatively easy to estimate the serum enzyme activity and can be applied for routine clinical use. The serum adenosine deaminase activity assayed by this method was elevated in patients with hepatic disease and the others, such as auto-immune diseases, rheumatoid arthritis, malignant lymphoma, leukemia and multiple myeloma. The presented experiments were designed to clarify the origin of the elevated adenosine deaminase in serum. The heat stability and pH optimum were found to be essentially identical in sera from hepatic disease and the others. However, Michael is constant (Km) for adenosine and V max were higher in the sera from patients with hepatic diseases than those of the others. The isoelectrofocusing pattern of the serum enzyme was shown quite different between hepatic disease and the others. The results in the present investigation indicated that the enhanced serum adenosine deaminase activity and its isoenzyme pattern were very useful for clinical diagnosis. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Hiroshima University School of Medicine |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
7182379 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0018-2052 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00664312 |
| 開始ページ |
|
|
開始ページ |
203 |
| 書誌情報 |
Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences
巻 31,
号 4,
p. 203-210,
発行日 1982-12-10
|
| 旧ID |
38371 |
HiroshimaJMedSci_31_203.pdf (4.8 MB)
