| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
DETERMINATION OF SERUM BILE ACIDS IN HEPATOBILIARY DISEASES BY GAS LIQUID CHROMATOGRAPHY : FASTING SERUM BILE ACID LEVELS AND ENDOGENOUS BILE ACID TOLERANCE TEST |
|
言語 |
en |
| タイトル |
|
|
タイトル |
ガスクロマトグラフィー法による肝胆道疾患の血清胆汁酸測定について : 空腹時血清胆汁酸および内因性胆汁酸負荷テスト |
|
言語 |
ja |
| 作成者 |
Kajiyama, Garo
Fukuhara, Choho
Oyamada, Ken
Horiuchi, Itaru
Nakagawa, Masahiro
Takata, Koki
Miyoshi, Akima
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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|
内容記述 |
The methods and results of a gas-liquid chromatographic analysis of bile acids in serum are presented. The analysis of bile acids in serum involves enzymatic hydrolysis (cholylglycine hydrolase), preparation of propionated methyl ester derivatives of bile acid and gas chromatographic procedure with 2.5 % OV-1. Adequate separation of the individual bile acids, (cholic, chenodeoxycholic and deoxycholic acid) was achieved with vitamine E caprylate as an internal standard. A detector response was linear and recovery of radioactive taurocholic acid and non-radioactive vitamine E caprylate added to the serum was 82.10±6.86 and 80.96±1.62% respectively. The serum fasting bile acid concentrations of normal controls were 3.17±2.34 for total bile acids, 1.17±1.25 for cholic acid, 1.34±2.11 for chenodeoxycholic acid and 1.29±0.95 μg/ml for deoxycholic acid. The differences in the serum total bile acid levels, magnitude of the increase in the serum concentration between cholic and chenodeoxycholic acid and serum concentration level of deoxycholic acid which were all characterized in various hepatobiliary diseases seemed to be useful for the diagnosis and differential diagnosis of hepatobiliary diseases. However, these serum bile acid concentrations were frequently observed overlapped in some of the individuals among hepatobiliary diseases. An endogenous bile add tolerance test with 2 μg/kg caerulein injection demonstrated more distinction in serum bile acid levels between normal and chronic active hepatitis and between chronic active hepatitis and liver cirrhosis than indicated by a fasting total bile acid level alone. Percent chenodeoxycholic acid increased more than any other individual bile acids during the endogenous bile acid tolerance test suggesting the most important role of chenodeoxycholic acid. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Hiroshima University School of Medicine |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
6765677 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0018-2052 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00664312 |
| 開始ページ |
|
|
開始ページ |
263 |
| 書誌情報 |
Hiroshima Journal of Medical Sciences
Hiroshima Journal of Medical Sciences
巻 30,
号 4,
p. 263-271,
発行日 1981-12-10
|
| 旧ID |
38400 |
HiroshimaJMedSci_30_263.pdf (5.3 MB)
