{"created":"2025-02-21T04:45:14.544384+00:00","id":2009105,"links":{},"metadata":{"_buckets":{"deposit":"cbf63f73-dc1e-46c2-946b-df83e796d508"},"_deposit":{"created_by":41,"id":"2009105","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2009105"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02009105","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Recombinant Protein Synthesis for Nanomaterial Assembly: Technical Overview","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中野, 綾菜","creatorNameLang":"ja"},{"creatorName":"Nakano, Ayana","creatorNameLang":"en"}],"familyNames":[{"familyName":"中野","familyNameLang":"ja"},{"familyName":"Nakano","familyNameLang":"en"}],"givenNames":[{"givenName":"綾菜","givenNameLang":"ja"},{"givenName":"Ayana","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"加藤, 功一","creatorNameLang":"ja"},{"creatorName":"Kato, Koichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"加藤","familyNameLang":"ja"},{"familyName":"Kato","familyNameLang":"en"}],"givenNames":[{"givenName":"功一","givenNameLang":"ja"},{"givenName":"Koichi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Recombinant DNA technology has emerged as a useful method for providing protein-based building blocks for nanomaterial assembly. This article provides an overview of the technical aspects of recombinant protein synthesis, especially for nanomaterial scientists and engineers. First, the rationale for the use of protein-based nanomaterials synthesized through recombinant DNA technology is presented. This article then focuses on simple standard protocols for protein expression in Escherichia coli. The last section presents several methods for creating artificial proteins with complex structures.","subitem_description_language":"en"},{"subitem_description":"This work was supported by Grant-in-Aid for Research Activity Start-up (No. 2l K2l0l4) and Fund for the Promotion of Joint Intemational Research (No. 19KK0278), JSPS.","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"ナノ学会"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"1347-8028","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"31"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"37","bibliographicPageStart":"31","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"ナノ学会会報"},{"bibliographic_title":"The bulletin of the Society of Nano Science and Technology"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"NanoGakkaiKaiho_20_31.pdf","filesize":[{"value":"273.2 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2009105/files/NanoGakkaiKaiho_20_31.pdf"},"version_id":"ec8fe068-e6f6-41be-b798-383be4459707"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"52826"},"item_title":"Recombinant Protein Synthesis for Nanomaterial Assembly: Technical Overview","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2009105","relation_version_is_last":true,"title":["Recombinant Protein Synthesis for Nanomaterial Assembly: Technical Overview"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-22T04:59:21.292639+00:00"}