{"created":"2025-02-21T04:40:54.649770+00:00","id":2008979,"links":{},"metadata":{"_buckets":{"deposit":"06018517-ee81-4e9f-a859-8b39d1e7bf62"},"_deposit":{"created_by":41,"id":"2008979","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2008979"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02008979","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Suppression of plasminogen activator inhibitor-1 by RNA interference attenuates pulmonary fibrosis","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Senoo, Tadashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Senoo","familyNameLang":"en"}],"givenNames":[{"givenName":"Tadashi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hattori, Noboru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hattori","familyNameLang":"en"}],"givenNames":[{"givenName":"Noboru","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tanimoto, Takuya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Tanimoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Takuya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Furonaka, Makoto","creatorNameLang":"en"}],"familyNames":[{"familyName":"Furonaka","familyNameLang":"en"}],"givenNames":[{"givenName":"Makoto","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishikawa, Nobuhisa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ishikawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Nobuhisa","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Fujitaka, Kazunori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Fujitaka","familyNameLang":"en"}],"givenNames":[{"givenName":"Kazunori","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Haruta, Yoshinori","creatorNameLang":"en"}],"familyNames":[{"familyName":"Haruta","familyNameLang":"en"}],"givenNames":[{"givenName":"Yoshinori","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Murai, Hiroshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Murai","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiroshi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yokoyama, Akihito","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yokoyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Akihito","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kohno, Nobuoki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kohno","familyNameLang":"en"}],"givenNames":[{"givenName":"Nobuoki","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2010 BMJ Publishing Group & British Thoracic Society"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Background and aim: There is a growing body of evidence demonstrating that plasminogen activator inhibitor-1 (PAI-1) is involved in the progression of pulmonary fibrosis. In fact, PAI-1 knockout mice are protected from bleomycin-induced pulmonary fibrosis. This study was conducted to determine whether the intrapulmonary administration of small interfering RNA (siRNA) targeting PAI-1 (PAI-1-siRNA) limits the development of bleomycin-induced pulmonary fibrosis.    Methods: Lung biopsies from patients with idiopathic pulmonary fibrosis (IPF) were stained for PAI-1. The distribution of siRNA in the lung, the PAI-1 level in bronchoalveolar (BAL) fluid and the extent of fibrotic changes in the lung were evaluated following the intranasal administration of PAI-1-siRNA in a mouse model of bleomycin-induced pulmonary fibrosis. The effect of PAI-1-siRNA on the epithelial to mesenchymal transition (EMT) was also evaluated using a mouse lung epithelial cell line, LA-4.    Results: PAI-1 was overexpressed in the hyperplastic type 2 pneumocytes lining the honeycomb lesions of patients with IPF. The single intranasal instillation of PAI-1-siRNA resulted in the diffuse uptake of siRNA into the epithelial cells lining the dense fibrotic lesions. The repeated administration of PAI-1-siRNA initiated during either the inflammatory or the fibrotic phase into bleomycin-injured mice reduced the PAI-1 level in BAL fluid and limited the accumulation of collagen in the lungs. EMT induced by transforming growth factor β (TGFβ) in LA-4 cells was inhibited by transfection with PAI-1-siRNA.    Conclusions: The direct suppression of PAI-1 in the lung by the intrapulmonary administration of PAI-1-siRNA attenuated the development and progression of pulmonary fibrosis. The inhibition of EMT may be, at least in part, involved in this effect.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"BMJ Publishing Group"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0040-6376","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00863090","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"334"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"340","bibliographicPageStart":"334","bibliographicVolumeNumber":"65","bibliographic_titles":[{"bibliographic_title":"Thorax"},{"bibliographic_title":"Thorax"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1136/thx.2009.119974","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1136/thx.2009.119974","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"Thorax_65_334.pdf","filesize":[{"value":"1.1 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2008979/files/Thorax_65_334.pdf"},"version_id":"45a658c9-3709-42b5-9208-ac3b2dadb529"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"30125"},"item_title":"Suppression of plasminogen activator inhibitor-1 by RNA interference attenuates pulmonary fibrosis","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2008979","relation_version_is_last":true,"title":["Suppression of plasminogen activator inhibitor-1 by RNA interference attenuates pulmonary fibrosis"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-22T04:41:50.773631+00:00"}