{"created":"2025-02-21T04:40:03.643908+00:00","id":2008951,"links":{},"metadata":{"_buckets":{"deposit":"44b3bfcd-eed7-4de5-a5c4-354296bee090"},"_deposit":{"created_by":41,"id":"2008951","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2008951"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02008951","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Suppression of inducible nitric oxide synthase and cyclooxygenase-2 gene expression by 22(R)-hydroxycholesterol requires de novo protein synthesis in activated macrophages","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yasuda, Toshimichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yasuda","familyNameLang":"en"}],"givenNames":[{"givenName":"Toshimichi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kanno, Masamoto","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kanno","familyNameLang":"en"}],"givenNames":[{"givenName":"Masamoto","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawamoto, Masashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kawamoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Masashi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yuge, Osafumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yuge","familyNameLang":"en"}],"givenNames":[{"givenName":"Osafumi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ninomiya, Yuichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ninomiya","familyNameLang":"en"}],"givenNames":[{"givenName":"Yuichi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2005 Elsevier Ltd"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"22(R)-Hydroxycholesterol","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammation","subitem_subject_scheme":"Other"},{"subitem_subject":"Macrophage","subitem_subject_scheme":"Other"},{"subitem_subject":"22R-HC","subitem_subject_scheme":"Other"},{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Liver X receptors (LXRs) play an important role in lipid metabolism. Recently, a role for these proteins was identified in suppressing the inflammatory response. However, it is not known whether the natural ligands of LXRs, e.g. 22(R)-hydroxycholesterol (22R-HC), can suppress the inflammatory response after the onset of inflammation. We demonstrate here that treatment of Lipopolysaccharide (LPS)-activated RAW264.7 macrophages with 22R-HC markedly suppressed nitric oxide (NO) production and inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression. Additionally, 22R-HC did not affect the DNA binding activity of NF-κB, AP-1 and C/EBP(s), important transcriptional factors for iNOS and COX-2 genes expression. Furthermore iNOS and COX-2 mRNA suppression by 22R-HC was diminished by cellular treatment with cycloheximide. These results suggest that 22R-HC suppresses the expression of iNOS and COX-2 genes through de novo protein synthesis of an unidentified protein in LPS-activated macrophages. © 2005 Elsevier Ltd. All rights reserved.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier Ltd"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0960-0760","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"376"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"383","bibliographicPageStart":"376","bibliographicVolumeNumber":"97","bibliographic_titles":[{"bibliographic_title":"The Journal of Steroid Biochemistry and Molecular Biology"},{"bibliographic_title":"The Journal of Steroid Biochemistry and Molecular Biology"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jsbmb.2005.06.030","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"16146692","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.jsbmb.2005.06.030","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"JSBMB_97_4_376.pdf","filesize":[{"value":"1.3 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2008951/files/JSBMB_97_4_376.pdf"},"version_id":"1a918733-eafc-4b25-821c-0a986614f3b9"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"14745"},"item_title":"Suppression of inducible nitric oxide synthase and cyclooxygenase-2 gene expression by 22(R)-hydroxycholesterol requires de novo protein synthesis in activated macrophages","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2008951","relation_version_is_last":true,"title":["Suppression of inducible nitric oxide synthase and cyclooxygenase-2 gene expression by 22(R)-hydroxycholesterol requires de novo protein synthesis in activated macrophages"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-22T04:39:11.118959+00:00"}