| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Enhancement of Osteogenesis by Concanavalin A in Human Bone Marrow Mesenchymal Stem Cell Cultures |
|
言語 |
en |
| 作成者 |
Sekiya, Kensuke
Nishimura, Masahiro
Suehiro, Fumio
Nishimura, Haruki
Hamada, Taizo
Kato, Yukio
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Concanavalin A |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Mesenchymal stem cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Calcification |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Osteogenesis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
This study investigates concanavalin A (ConA) as a novel factor that may enhance osteogenesis of mesenchymal stem cells (MSCs) in vitro. Various factors have been studied as promoting factors for MSC osteogenesis in vivo and in vitro. However, their safety, effectiveness, and cost may not be ideal. So, human MSCs were cultured in osteogenic medium in the presence or absence of ConA. We used calcium assays to compare the effects of ConA and BMP-2 on MSC calcification. Also enzyme-linked immunosorbent assay (ELISA) and quantitative PCR were used to evaluate the expression levels of bone specific markers. ConA was observed to enhance the calcification and this effect was comparable to that of BMP-2. Combination of ConA and BMP-2 further enhanced the calcification slightly but significantly. ConA also increased osteocalcin and BMP-2 protein levels in the medium of MSC cultures. Furthermore, ConA increased osteocalcin, RUNX2, BMP-2, and BMP-4 mRNA expression levels. However, gene expression pattern of MSCs stimulated by ConA was different from that observed with BMP-2. These results, taken together, suggest that ConA and BMP-2 enhance MSC osteogenesis via different pathways. The ConA-induced bone formation in MSC cultures may be useful in regenerative medicine or tissue engineering in clinical studies and in basic research on bone formation. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Wichtig Editore |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0391-3988 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00679932 |
| 開始ページ |
|
|
開始ページ |
708 |
| 書誌情報 |
The International Journal of Artificial Organs
The International Journal of Artificial Organs
巻 31,
号 8,
p. 708-715,
発行日 2008-08
|
| 旧ID |
25916 |
IntJArtifOrgans_31_708.pdf (556.8 KB)
