{"created":"2025-02-21T04:32:28.236479+00:00","id":2008707,"links":{},"metadata":{"_buckets":{"deposit":"80dc7c95-935b-4833-abc4-7a607fe00b59"},"_deposit":{"created_by":41,"id":"2008707","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2008707"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02008707","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Structural and functional insights into thermally stable cytochrome c' from a thermophile","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujii, Sotaro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Fujii","familyNameLang":"en"}],"givenNames":[{"givenName":"Sotaro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oki, Hiroya","creatorNameLang":"en"}],"familyNames":[{"familyName":"Oki","familyNameLang":"en"}],"givenNames":[{"givenName":"Hiroya","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawahara, Kazuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kawahara","familyNameLang":"en"}],"givenNames":[{"givenName":"Kazuki","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamane, Daisuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamane","familyNameLang":"en"}],"givenNames":[{"givenName":"Daisuke","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamanaka, Masaru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamanaka","familyNameLang":"en"}],"givenNames":[{"givenName":"Masaru","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Maruno, Takahiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Maruno","familyNameLang":"en"}],"givenNames":[{"givenName":"Takahiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kobayashi, Yuji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kobayashi","familyNameLang":"en"}],"givenNames":[{"givenName":"Yuji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Masanari, Misa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Masanari","familyNameLang":"en"}],"givenNames":[{"givenName":"Misa","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Wakai, Satoshi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Wakai","familyNameLang":"en"}],"givenNames":[{"givenName":"Satoshi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nishihara, Hirofumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Nishihara","familyNameLang":"en"}],"givenNames":[{"givenName":"Hirofumi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohkubo, Tadayasu","creatorNameLang":"en"}],"familyNames":[{"familyName":"Ohkubo","familyNameLang":"en"}],"givenNames":[{"givenName":"Tadayasu","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sambongi, Yoshihiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sambongi","familyNameLang":"en"}],"givenNames":[{"givenName":"Yoshihiro","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2017 The Authors Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"protein stability","subitem_subject_scheme":"Other"},{"subitem_subject":"cytochrome c","subitem_subject_scheme":"Other"},{"subitem_subject":"thermophile","subitem_subject_scheme":"Other"},{"subitem_subject":"protein structure","subitem_subject_scheme":"Other"},{"subitem_subject":"mutagenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"ligand-binding","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Thermophilic Hydrogenophilus thermoluteolus cytochrome c0 (PHCP) exhibits higher thermal stability than a mesophilic counterpart, Allochromatium vinosum cytochrome c0 (AVCP), which has a homo-dimeric structure and ligand-binding ability. To understand the thermal stability mechanism and ligand-binding ability of the thermally stable PHCP protein, the crystal structure of PHCP was first determined. It formed a homo-dimeric structure, the main chain root mean square deviation (rmsd) value between PHCP and AVCP being 0.65 A ° . In the PHCP structure, six specific residues appeared to strengthen the heme-related and subunit–subunit interactions, which were not conserved in the AVCP structure. PHCP variants having altered subunit–subunit interactions were more severely destabilized than ones having altered heme-related interactions. The PHCP structure further revealed a ligand-binding channel and a penta-coordinated heme, as observed in the AVCP protein. A spectroscopic study clearly showed that some ligands were bound to the PHCP protein. It is concluded that the dimeric PHCP from the thermophile is effectively stabilized through heme-related and subunit–subunit interactions with conservation of the ligand-binding ability.","subitem_description_language":"en"},{"subitem_description":"This work was performed under the Cooperative Research Program of the “Network Joint Research Center for Materials and Devices\".","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Wiley"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0961-8368","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1469-896X","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"737"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"748","bibliographicPageStart":"737","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Protein Science"},{"bibliographic_title":"Protein Science"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/pro.3120","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28097774","subitem_relation_type_select":"PMID"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/pro.3120","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"ProteinSci_26_737.pdf","filesize":[{"value":"664.6 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2008707/files/ProteinSci_26_737.pdf"},"version_id":"af6d20a4-c4f3-4923-bc94-910941d194a8"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"48606"},"item_title":"Structural and functional insights into thermally stable cytochrome c' from a thermophile","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2008707","relation_version_is_last":true,"title":["Structural and functional insights into thermally stable cytochrome c' from a thermophile"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-22T04:08:35.296777+00:00"}