{"created":"2025-02-21T04:02:16.512562+00:00","id":2007764,"links":{},"metadata":{"_buckets":{"deposit":"d3933bfc-ffe5-4abe-932d-8f9a7de0a378"},"_deposit":{"created_by":41,"id":"2007764","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007764"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007764","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Effect of transforming growth factor-β1 on functional expression of monocarboxylate transporter 1 in alveolar epithelial A549 cells","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uddin, Mohi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Uddin","familyNameLang":"en"}],"givenNames":[{"givenName":"Mohi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawami, Masashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kawami","familyNameLang":"en"}],"givenNames":[{"givenName":"Masashi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yumoto, Ryoko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yumoto","familyNameLang":"en"}],"givenNames":[{"givenName":"Ryoko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takano, Mikihisa","creatorNameLang":"en"}],"familyNames":[{"familyName":"Takano","familyNameLang":"en"}],"givenNames":[{"givenName":"Mikihisa","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"This is a post-peer-review, pre-copyedit version of an article published in  Naunyn-Schmiedeberg's Archives of Pharmacology. The final authenticated version is available online at: https://doi.org/10.1007/s00210-019-01802-3"},{"subitem_rights":"This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Alveolar epithelial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Epithelial-mesenchymal transition","subitem_subject_scheme":"Other"},{"subitem_subject":"γ-hydroxybutyrate","subitem_subject_scheme":"Other"},{"subitem_subject":"Monocarboxylate transporter 1","subitem_subject_scheme":"Other"},{"subitem_subject":"Transforming growth factor-β1","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Epithelial-mesenchymal transition (EMT) contributes to the development of severe lung diseases, such as pulmonary fibrosis. Recently, it has been reported that EMT involves complex metabolic reprogramming triggered by several factors including transforming growth factor (TGF-β1) and that monocarboxylate transporter (MCT1) plays an essential role in these metabolic changes. The aim of the present study was to clarify the functional expression of MCT1 during TGF-β1-induced EMT in alveolar epithelial A549 cells. The transport function of MCT1 in A549 cells was examined using [3H]γ-hydroxybutyrate (GHB) and [3H] lactic acid (LA) as substrates and α-cyano-4-hydroxycinnamate (CHC), lactic acid, phloretin, and AR-C155858 (AR) as inhibitors of MCT1. EMT was induced by treating the cells with TGF-β1. mRNA and protein expression levels were analyzed using real-time PCR and Western blotting, respectively. Time-, temperature-, and pH-dependent GHB and LA uptake were observed in A549 cells. CHC, lactic acid, phloretin, and AR significantly inhibited the uptake of GHB in a concentration-dependent manner, suggesting that MCT1 is primarily responsible for transport of monocarboxylates such as GHB and LA in A549 cells. TGF-β1 treatment significantly enhanced GHB and LA uptake as well as the mRNA and protein expression levels of MCT1 in A549 cells. These changes were neutralized by co-treatment with SB431542, an inhibitor for the TGF-β1 signaling pathway. CHC and AR had no effect on TGF-β1-induced EMT-related gene expression changes. Here, we have clearly characterized functional expression of MCT1 in A549 cells and have shown that MCT1 may be upregulated via the TGF-β1 signaling pathway.","subitem_description_language":"en"},{"subitem_description":"This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Sciences (JSPS) (numbers; 26293033, 15 K08074, and 16 K18945).","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Springer"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0028-1298","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1432-1912","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"889"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"896","bibliographicPageStart":"889","bibliographicVolumeNumber":"393","bibliographic_titles":[{"bibliographic_title":"Naunyn-Schmiedeberg's Archives of Pharmacology"},{"bibliographic_title":"Naunyn-Schmiedeberg's Archives of Pharmacology"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00210-019-01802-3","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"31900520","subitem_relation_type_select":"PMID"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00210-019-01802-3","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-06-01"}],"displaytype":"simple","fileDate":[{"fileDateType":"Available","fileDateValue":"2021-06-01"}],"filename":"NaunynSchmiedebergsArchPharmacol_393_889.pdf","filesize":[{"value":"818.8 KB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007764/files/NaunynSchmiedebergsArchPharmacol_393_889.pdf"},"version_id":"4d03724d-94b1-4637-bfe7-d824acb228db"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"50464"},"item_1732772494514":{"attribute_name":"備考","attribute_value":"Post-print version/PDF may be used in an institutional repository after an embargo period of 12 months."},"item_title":"Effect of transforming growth factor-β1 on functional expression of monocarboxylate transporter 1 in alveolar epithelial A549 cells","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007764","relation_version_is_last":true,"title":["Effect of transforming growth factor-β1 on functional expression of monocarboxylate transporter 1 in alveolar epithelial A549 cells"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T10:56:14.104675+00:00"}