| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Effect of transforming growth factor-β1 on functional expression of monocarboxylate transporter 1 in alveolar epithelial A549 cells |
|
言語 |
en |
| 作成者 |
Uddin, Mohi
Kawami, Masashi
Yumoto, Ryoko
Takano, Mikihisa
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
This is a post-peer-review, pre-copyedit version of an article published in Naunyn-Schmiedeberg's Archives of Pharmacology. The final authenticated version is available online at: https://doi.org/10.1007/s00210-019-01802-3 |
| 権利情報 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Alveolar epithelial cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Epithelial-mesenchymal transition |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
γ-hydroxybutyrate |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Monocarboxylate transporter 1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Transforming growth factor-β1 |
| 内容記述 |
|
|
内容記述 |
Epithelial-mesenchymal transition (EMT) contributes to the development of severe lung diseases, such as pulmonary fibrosis. Recently, it has been reported that EMT involves complex metabolic reprogramming triggered by several factors including transforming growth factor (TGF-β1) and that monocarboxylate transporter (MCT1) plays an essential role in these metabolic changes. The aim of the present study was to clarify the functional expression of MCT1 during TGF-β1-induced EMT in alveolar epithelial A549 cells. The transport function of MCT1 in A549 cells was examined using [3H]γ-hydroxybutyrate (GHB) and [3H] lactic acid (LA) as substrates and α-cyano-4-hydroxycinnamate (CHC), lactic acid, phloretin, and AR-C155858 (AR) as inhibitors of MCT1. EMT was induced by treating the cells with TGF-β1. mRNA and protein expression levels were analyzed using real-time PCR and Western blotting, respectively. Time-, temperature-, and pH-dependent GHB and LA uptake were observed in A549 cells. CHC, lactic acid, phloretin, and AR significantly inhibited the uptake of GHB in a concentration-dependent manner, suggesting that MCT1 is primarily responsible for transport of monocarboxylates such as GHB and LA in A549 cells. TGF-β1 treatment significantly enhanced GHB and LA uptake as well as the mRNA and protein expression levels of MCT1 in A549 cells. These changes were neutralized by co-treatment with SB431542, an inhibitor for the TGF-β1 signaling pathway. CHC and AR had no effect on TGF-β1-induced EMT-related gene expression changes. Here, we have clearly characterized functional expression of MCT1 in A549 cells and have shown that MCT1 may be upregulated via the TGF-β1 signaling pathway. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Sciences (JSPS) (numbers; 26293033, 15 K08074, and 16 K18945). |
| 出版者 |
|
|
出版者 |
Springer |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s00210-019-01802-3 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
31900520 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s00210-019-01802-3 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0028-1298 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1432-1912 |
| 開始ページ |
|
|
開始ページ |
889 |
| 書誌情報 |
Naunyn-Schmiedeberg's Archives of Pharmacology
Naunyn-Schmiedeberg's Archives of Pharmacology
巻 393,
号 5,
p. 889-896,
発行日 2020-05
|
| 旧ID |
50464 |
| 備考 |
Post-print version/PDF may be used in an institutional repository after an embargo period of 12 months. |
NaunynSchmiedebergsArchPharmacol_393_889.pdf (818.8 KB)
