{"created":"2025-02-21T04:01:32.673254+00:00","id":2007739,"links":{},"metadata":{"_buckets":{"deposit":"4435b81c-2fe6-4021-8316-cc1977c5598d"},"_deposit":{"created_by":41,"id":"2007739","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007739"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007739","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Phosphate-binding tag : A new tool to visualize phosphorylated proteins","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kinoshita, Eiji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita","familyNameLang":"en"}],"givenNames":[{"givenName":"Eiji","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinoshita-Kikuta, Emiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"Kinoshita-Kikuta","familyNameLang":"en"}],"givenNames":[{"givenName":"Emiko","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takiyama, Kei","creatorNameLang":"en"}],"familyNames":[{"familyName":"Takiyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Kei","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koike, Tohru","creatorNameLang":"en"}],"familyNames":[{"familyName":"Koike","familyNameLang":"en"}],"givenNames":[{"givenName":"Tohru","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2006 by the American Society for Biochemistry and Molecular Biology."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"Phosphoproteomics","subitem_subject_scheme":"Other"},{"subitem_subject":"Electroblotting analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"Chemiluminescence detection","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphate-affinity electrophoresis","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphorylated protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphorylation","subitem_subject_scheme":"Other"},{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"We introduce two methods for the visualization of phosphorylated proteins using alkoxide-bridged dinuclear metal (i.e., Zn2+ or Mn2+) complexes as novel phosphate-binding tag (Phos-tag) molecules. Both Zn2+. and Mn2+.Phos-tag molecules preferentially capture phosphomonoester dianions bound to Ser, Thr, and Tyr residues. One method is based on an enhanced chemiluminescence (ECL) system using biotin-pendant Zn2+.Phos-tag and horseradish peroxidase-conjugated streptavidin (HRP.SA). We demonstrate the electroblotting analyses of protein phosphorylation status by the phosphate-selective ECL signals. Another method is based on the mobility shift of phosphorylated proteins in SDS-PAGE with polyacrylamide-bound Mn2+.Phos-tag. Phosphorylated proteins in the gel are visualized as slower migration bands compared with corresponding dephosphorylated proteins. We demonstrate the kinase and phosphatase assays by the phosphate-affinity electrophoresis (Mn2+.Phos-tag SDS-PAGE).","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"1535-9476","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11789524","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"749"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"757","bibliographicPageStart":"749","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Molecular and Cellular Proteomics"},{"bibliographic_title":"Molecular and Cellular Proteomics"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/mcp.T500024-MCP200","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"16340016","subitem_relation_type_select":"PMID"}},{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1074/mcp.T500024-MCP200","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"MCP_5_749.pdf","filesize":[{"value":"2.4 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007739/files/MCP_5_749.pdf"},"version_id":"6337864b-17af-4319-8fcc-9177e0615541"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"17109"},"item_title":"Phosphate-binding tag : A new tool to visualize phosphorylated proteins","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007739","relation_version_is_last":true,"title":["Phosphate-binding tag : A new tool to visualize phosphorylated proteins"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T10:50:24.492184+00:00"}