| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system and induces an up-regulation of anti-apoptotic NF-κB target genes protecting the macrophage from staurosporine-induced apoptosis |
|
言語 |
en |
| 作成者 |
Okuda, Jun
Arikawa, Yoko
Takeuchi, Yusuke
Mostafa, Mahmoud
Suzaki, Etsuko
Kataoka, Katsuko
Suzuki, Toshiharu
Okinaka, Yasushi
Nakai, Toshihiro
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Edwardsiella tarda |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
the type III secretion system |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
NF-кB |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
anti-apoptosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
intracellular replication |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
macrophage |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. Resistance to phagocytic killing may be involved in the pathogenicity of this bacterium. Here we show that intracellular replication of E. tarda in murine macrophages is dependent on the type III secretion system and induces an anti-apoptotic effect by up-regulating anti-apoptotic NF-κB target genes. The wild-type strain replicates within the phagosomal membrane of macrophages; whereas the type III mutant does not. Microarray analysis shows the mRNA expression level of NF-κB target genes (e.g. pro-inflammatory cytokines and anti-apoptotic genes) in macrophages infected with the wild-type strain were up-regulated compared to macrophages infected with the type III mutant. Up-regulation of Bcl2a1a, Bcl2a1b, cIAP-2, and TRAF1 genes induced expression of anti-apoptotic proteins to protect macrophages from apoptosis induced by staurosporine. Further, this protection was inhibited by adding kamebakaurin, an inhibitor of NF-κB activation and was confirmed using an NF-κB reporter gene assay. Up-regulation of anti-apoptotic NF-κB target genes is responsible for the anti-apoptotic activity of E. tarda and is required for intracellular replication in murine macrophages. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.micpath.2006.08.002 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
17049431 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.micpath.2006.08.002 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0882-4010 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10609161 |
| 開始ページ |
|
|
開始ページ |
226 |
| 書誌情報 |
Microbial Pathogenesis
Microbial Pathogenesis
巻 41,
号 6,
p. 226-240,
発行日 2006-12
|
| 旧ID |
17108 |
MP_41_226.pdf (1.7 MB)
