| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Enrichment Using an Up-flow Column Reactor and Community Structure of Marine Anammox Bacteria from Coastal Sediment |
|
言語 |
en |
| 作成者 |
Kindaichi, Tomonori
Awata, Takanori
Suzuki, Yuji
Tanabe, Katsuichiro
Hatamoto, Masashi
Ozaki, Noriatsu
Ohashi, Akiyoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
anaerobic ammonium oxidation (anammox) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
marine anammox bacteria |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
“Candidatus Scalindua" species |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
enrichment culture |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
FISH |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
phylogenetic analysis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
519 |
| 内容記述 |
|
|
内容記述 |
We established an enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria using an up-flow column reactor fed with artificial sea water supplemented with nitrogen and minerals and inoculated with coastal surface sediment collected from Hiroshima Bay. After 2 months of reactor operation, simultaneous removal of NH4+ and NO2- was observed, suggesting that an anammox reaction was proceeding. A total nitrogen removal rate of 2.17 g-N L-1 day-1 was attained on day 594 while the nitrogen loading rate was 3.33 g-N L-1 day-1. Phylogenetic analysis revealed that at least two dominant “Candidatus Scalindua" species were present in this reactor. Moreover, many uncultured bacteria and archaea, including candidate division or ammonia-oxidizing archaea, were present. Fluorescence in situ hybridization (FISH) revealed that anammox bacteria accounted for 85.5 ± 4.5% of the total bacteria at day 393. We also designed two oligonucleotide probes specific to each dominant “Candidatus Scalindua" species. A simultaneous FISH analysis using both probes showed that two different “Candidatus Scalindua" species were clearly recognizable and coexisted during reactor operation, although there was some variation in their abundance. The marine anammox bacteria enriched in this study have potential applications to the treatment of industrial wastewater containing high levels of ammonium and salt. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
日本微生物生態学会 |
| 出版者 |
|
|
出版者 |
日本土壌微生物学会 |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1264/jsme2.ME10158 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1264/jsme2.ME10158 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1342-6311 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11551577 |
| 開始ページ |
|
|
開始ページ |
67 |
| 書誌情報 |
Microbes and Environments
Microbes and Environments
巻 26,
号 1,
p. 67-73,
発行日 2011
|
| 旧ID |
34541 |
Microbes-and-Environments26_67.pdf (799.3 KB)
