| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG(+) phenotype, lack of virulence and absence of a capsule |
|
言語 |
en |
| 作成者 |
Kawanishi, Michiko
Yoshida, Teruyoyo
Kijima, Mayumi
Yagyu, Kazuhiro
Nakai, Toshihiro
Okada, Sanae
Endo, Akihito
Murakami, Masaru
Morita, Hidetoshi
Suzuki, Shoko
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| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
Copyright (c) 2007 Authors |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Broccoli sprout |
| 主題 |
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主題Scheme |
Other |
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主題 |
Japan |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Lactococcus garvieae |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Phage and pulsed-field gel electrophoresis |
| 主題 |
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主題Scheme |
Other |
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主題 |
Phathogenicity |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Radish sprouts |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Seriola |
| 主題 |
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|
主題Scheme |
NDC |
|
主題 |
600 |
| 内容記述 |
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|
内容記述 |
Aims: To identify Lactococcus garvieae isolates from radish and broccoli sprouts and compare them with virulent and less virulent mutant strains obtained from yellowtails with regard to KG phenotype, presence of a capsule and virulence towards yellowtails and mice. Methods and Results: Comparative 16S rRNA gene sequence analysis of six isolates obtained from radish and broccoli sprouts indicated that they were L. garvieae (similarity >99%). They were compared with KG9502, Lg2 and ATCC49156 strains obtained from yellowtails. A less virulent mutant strain Lg2-S was obtained by Lg2 subculture. Biochemical characterization of the six strains resembled that of KG9502, Lg2, ATCC49156 and Lg2-S, except for saccharose and tagatose acidification and the presence of hippuricase. These six strains were nonpathogenic towards yellowtails and mice, nonsusceptible to bacteriophages and demonstrated heterogeneity on pulsed-field gel electrophoresis analysis. Using transmission electron microscopy, a capsule was observed in KG9502 and Lg2 but not in ATCC49156 and Lg2-S. Conclusions: We isolated L. garvieae strains that lacked pathogenicity towards yellowtails and mice from radish and broccoli sprouts; these were noncapsulated and exhibited KG+ phenotype. Significance and Impact of the Study: This is the first documentation of L. garvieae isolated from terrestrial plants. These isolates exhibited genetic diversity; however, they were noncapsulated and nonpathogenic towards yellowtails and mice. |
|
言語 |
en |
| 出版者 |
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出版者 |
Blackwell |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
関連名称 |
The definitive version is available at www.blackwell-synergy.com |
| 関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/j.1472-765X.2007.02114.x |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1111/j.1472-765X.2007.02114.x |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0266-8254 |
| 収録物識別子 |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10462245 |
| 開始ページ |
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|
開始ページ |
481 |
| 書誌情報 |
Letters in Applied Microbiology
Letters in Applied Microbiology
巻 44,
号 5,
p. 481-487,
発行日 2007-05
|
| 旧ID |
20784 |
LettApplMicrobiol_44_481.pdf (653.3 KB)
