| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
The YLDL sequence within Sendai virus m protein is critical for budding of virus-like particles and interacts with Alix/AIP1 independently of C protein |
|
言語 |
en |
| 作成者 |
Irie, Takashi
Shimazu, Yukie
Yoshida, Tetsuya
Sakaguchi, Takemasa
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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権利情報 |
Copyright (c) 2007 American Society for Microbiology |
| 主題 |
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主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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内容記述 |
For many enveloped viruses, cellular multivesicular body (MVB) sorting machinery has been reported to be utilized for efficient viral budding. Matrix and Gag proteins have been shown to contain one or two L-domain motifs (PPxY, PT/SAP, YPDL, and FPIV), some of which interact specifically with host cellular proteins involved in multivesicular body sorting, which are recruited to the viral budding site. However, for many enveloped viruses, L-domain motifs have not yet been identified, and the involvement of MVB sorting machinery in viral budding is still unknown. Here we show that both Sendai virus (SeV) matrix protein M and accessory protein C contribute to virus budding by physically interacting with Alix/AIP1. A YLDL sequence within the M protein showed L-domain activity, and its specific interaction with the N-terminus of Alix/AIP1 (1-211) was important for the budding of virus-like particles (VLPs) of M protein. In addition, M-VLP budding was inhibited by the overexpression of some deletion mutants of Alix/AIP1 and depletion of endogenous Alix/AIP1 using specific siRNAs. The YLDL sequence was not replaceable by other L-domain motifs, such as PPxY and PT/SAP, and even YPxL. C protein was also able to physically interact with the N-terminus of Alix/AIP1 (212-357) and enhanced M-VLP budding independently of M-Alix/AIP1 interaction, although it was not released from the transfected cells itself. Our results suggest that the interaction of multiple viral proteins with Alix/AIP1 may enhance the efficiency of the utilization of cellular MVB sorting machinery for efficient SeV budding. |
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言語 |
en |
| 出版者 |
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出版者 |
American Society for Microbiology |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
AO |
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出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1128/JVI.02218-06 |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
http://dx.doi.org/10.1128/JVI.02218-06 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-538X |
| 収録物識別子 |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00708779 |
| 開始ページ |
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開始ページ |
2263 |
| 書誌情報 |
Journal of Virology
Journal of Virology
巻 81,
号 5,
p. 2263-2273,
発行日 2007-03
|
| 旧ID |
20596 |
JVI_81-5_2263.pdf (248.0 KB)
