| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) has potential tumour-suppressive activity in human lung cancer |
|
言語 |
en |
| 作成者 |
Chen, Yuan
Ye, Fei
Pacyana-Gengelbach, Manuela
Knösel, Thomas
Deutschmann, Nicole
Lund Per
Yasumoto, Hiroaki
Usui, Tsuguru
Petersen, Iver
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
Copyright (c) 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
IGFBP-rP1 |
| 主題 |
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主題Scheme |
Other |
|
主題 |
human lung cancer |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
DNA methylation |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
stable transfection |
| 主題 |
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主題Scheme |
Other |
|
主題 |
tumour suppressor |
| 主題 |
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|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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|
内容記述 |
Insulin-like growth binding protein-related protein 1 (IGFBP-rP1) has decreased expression in various tumors, but the role of IGFBP-rP1 in lung cancer has not yet been elucidated. In this study, we evaluated the IGFBP-rP1 expression in lung cancer cell lines and we found a reduced expression of IGFBP-rP1 at both mRNA and protein levels. In a tissue microarray containing 138 primary tumors analyzed by immunohistochemistry, 58 cases (42%) exhibited no expression of IGFBP-rP1, additionally, an association between IGFBP-rP1 expression and tumor gradings was found in squamous cell lung cancer. Neither deletion or gene rearrangement nor loss of heterozigosity was responsible for the inactivation of IGFBP-rP1. However, 5-aza-2'-deoxycytidine treatment restored the expression of IGFBP-rP1 in 3 out of 4 lung cancer cell lines. Sequencing of sodium bisulfite?treated genomic DNA from these 3 lung cancer cell lines revealed a heterogeneous methylation pattern in the region of exon 1 and intron 1. Stable transfection of IGFBP-rP1 full-length cDNA into a lung cancer cell line H2170 led to an increased protein expression of IGFBP-rP1. IGFBP-rP1 positive transfectants remarkably reduced the ability of colony formation in soft agar, suppressed the tumor growth rate in nude mice and increased the number of apoptotic cells as well as the expression level of casepase-3 compared to controls. Moreover, treatment with differentiation modulating agent 5-bromodeoxyuridine (BrdU) led to an enhanced expression of IGFBP-rP1 in lung cancer cells. Out data suggest that IGFBP-rP1 is a tumor suppressor possibly via DNA methylation and induction of apoptosis in human lung cancer. |
|
言語 |
en |
| 出版者 |
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|
出版者 |
John Wiley & Sons, Ltd. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1002/path.2132 |
| 関連情報 |
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|
識別子タイプ |
PMID |
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関連識別子 |
17236181 |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1002/path.2132 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0022-3417 |
| 収録物識別子 |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00704326 |
| 開始ページ |
|
|
開始ページ |
431 |
| 書誌情報 |
Journal of Pathology
Journal of Pathology
巻 211,
号 4,
p. 431-438,
発行日 2007-01
|
| 旧ID |
20781 |
JPathol_211_431.pdf (1017.5 KB)
