| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Identification of prostatic secreted proteins in mice by mass spectrometric analysis and evaluation of lobe-specific and androgen-dependent mRNA expression |
|
言語 |
en |
| 作成者 |
Fujimoto, Nariaki
Akimoto, Yukimi
Suzuki, Tomoharu
Kitamura, Shigeyuki
Ohta, Shigeru
|
| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
Copyright (c) 2006 Elsevier Ltd. |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
mouse prostate |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
secretion |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
proteome analysis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
lobe specific |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
androgen regulation |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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|
内容記述 |
Rats and guinea pigs have frequently been used to study the development of the prostate and the mechanism of androgen action, but the mouse prostate has also become an attractive model for prostate research, because an enormous range of genetically altered mice is now available. However, the secretion of proteins in the mouse prostate has not yet been well investigated. In the present study, major secreted proteins from the ventral (VP), dorso-lateral (DLP) and anterior prostate (AP) of mice were identified by means of two-dimensional gel electrophoresis followed by MALDI-TOF mass spectrometric analysis. A quantitative RT-PCR method was further employed to examine the androgen-dependent transcriptional regulation of the identified proteins. Proteome analysis revealed that the VP secretes spermine binding protein, serine protease inhibitor Kazal type 3 and a 91 kDa hypothetical scavenger receptor (AK035662). The DLP and the AP secrete a protein similar to immunoglobulin binding protein (IgBPLP) and one of the experimental autoimmune prostatitis antigen proteins (EAPA2). Peroxiredoxin-6, glucose-regulated protein 78, zinc-a2-glycoprotein and phospholipase Ca are also secreted. Castration of animals led to a decrease in the mRNAs of these secreted proteins, although the extents of change varied greatly among different lobes. We present here an overview of mouse prostate secretion, which should contribute to an understanding of the biological functions of the prostate gland, as well as the androgen-dependency of prostate secretion. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1677/joe.1.06733 |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
17003280 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1677/joe.1.06733 |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0022-0795 |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00697140 |
| 開始ページ |
|
|
開始ページ |
793 |
| 書誌情報 |
Journal of Endocrinology
Journal of Endocrinology
巻 190,
号 3,
p. 793-803,
発行日 2006-09
|
| 旧ID |
17091 |
JOE_190_793.pdf (1020.4 KB)
