{"created":"2025-02-21T03:45:47.071983+00:00","id":2007234,"links":{},"metadata":{"_buckets":{"deposit":"a1b515bc-03ff-49a5-97f3-9bb9a96891bb"},"_deposit":{"created_by":41,"id":"2007234","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2007234"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02007234","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Primary Structure and Carbohydrate Binding Specificity of a Potent Anti-HIV Lectin Isolated from the Filamentous Cyanobacterium Oscillatoria agardhii","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Yuichiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"Sato","familyNameLang":"en"}],"givenNames":[{"givenName":"Yuichiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Okuyama, Satomi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Okuyama","familyNameLang":"en"}],"givenNames":[{"givenName":"Satomi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hori, Kanji","creatorNameLang":"en"}],"familyNames":[{"familyName":"Hori","familyNameLang":"en"}],"givenNames":[{"givenName":"Kanji","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2007 by the American Society for Biochemistry and Molecular Biology."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"470","subitem_subject_scheme":"NDC"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The primary structure of a lectin,designated OAA, isolated from thefreshwater cyanobacterium, Oscillatoriaagardhii NIES-204, was determined by thecombination of Edman degradation andESI-mass spectrometry. OAA is apolypeptide (MW 13,925) consisting of twotandem repeats. Interestingly, each repeatsequence of OAA showed a high degree ofsimilarity to those of a myxobacterium,Myxococcus xanthus hemagglutinin(MBHA), and a marine red alga Eucheumaserra lectin (ESA-2). A systematic bindingassay with pyridylaminated oligosaccharidesrevealed that OAA exclusively binds to highmannose (HM) type N-glycans, but not toother N-glycans, including complex types,hybrid types and the pentasaccharide core,or oligosaccharides from glycolipids. OAAdid not interact with any of free mono-andoligomannoses that are constituents of thebranched oligomannosides. These resultssuggest that the core disaccharide, GlcNAc-GlcNAc, is also essential for binding to OAA.The binding activity of OAA to HMtype N-glycanswas dramatically decreased whenα1-2 Man was attached to α1-3 Manbranched from the α1-6 Man of thepentasaccharide core. This specificity ofOAA for HM type oligosaccharides isdistinct from other HM-binding lectins.Kinetic analysis with an HMheptasaccharide revealed that OAApossesses two carbohydrate-binding sitesper molecule, with an association constant of2.41×10^8M^-1. Furthermore, OAA potentlyinhibits HIV replication in MT-4 cells(EC50=44.5 nM). Thus, we have found anovel lectin family sharing similar structureand carbohydrate binding specificity amongbacteria, cyanobacteria, and marine algae.","subitem_description_language":"en"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The American Society for Biochemistry and Molecular Biology"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00251083","subitem_source_identifier_type":"NCID"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"11021"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-04-13","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageEnd":"11029","bibliographicPageStart":"11021","bibliographicVolumeNumber":"282","bibliographic_titles":[{"bibliographic_title":"Journal of Biological Chemistry"},{"bibliographic_title":"Journal of Biological Chemistry"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_b1a7d7d4d402bcce","subitem_version_type":"AO"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M701252200","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1074/jbc.M701252200","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"JBC_282_11021.pdf","filesize":[{"value":"1.6 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2007234/files/JBC_282_11021.pdf"},"version_id":"c61d2e81-4a6a-4382-bdba-837510de06fe"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"21539"},"item_title":"Primary Structure and Carbohydrate Binding Specificity of a Potent Anti-HIV Lectin Isolated from the Filamentous Cyanobacterium Oscillatoria agardhii","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2007234","relation_version_is_last":true,"title":["Primary Structure and Carbohydrate Binding Specificity of a Potent Anti-HIV Lectin Isolated from the Filamentous Cyanobacterium Oscillatoria agardhii"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T09:29:49.888092+00:00"}