| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW 264.7 in the presence of the toll-like receptor 4 ligand bacterial endotoxin |
|
言語 |
en |
| 作成者 |
Yamashita, Akiko
Soga, Yoshihiko
Iwamoto, Yoshihiro
Asano, T.
Li, Y.
Abiko, Yoshimitsu
Nishimura, Fusanori
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2009 Nature Publishing Group |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
adipocyte-macrophage interaction |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
TLR-4; endotoxin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
gene expression |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
microarray analyses |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Recent studies have suggested macrophages were integrated to adipose tissues to interact with adipocytes, thereby exacerbating inflammatory responses. Furthermore, both adipocytes and macrophages appear to express TLR-4, and free fatty acids may stimulate cells via TLR-4. Herein, we analyzed genes differentially expressed in adipocytes when co-cultured with macrophages in the presence of a ligand for TLR-4, bacterial lipopolysaccharide. RAW264.7, a murine macrophage cell line and differentiated 3T3-L1 adipocytes were co-cultured using a transwell system. Genes differentially expressed in adipocytes were analyzed by the DNA microarray method following 4, 8, 12 and 24h stimulation with 1 ng/ml of E. coli LPS. Randomly selected genes with high expressions were confirmed by quantitative methods at both the gene and the protein level. Co-culture of macrophages and adipocytes with a low LPS concentration (1 ng/ml) markedly up-regulated gene expressions associated with inflammation and/or angiogenesis, such as those of IL-6, MCP-1, RANTES and CXCL1/KC, in adipocytes. Furthermore, several genes associated with insulin resistance were differentially expressed. Up-regulations of genes encoding MCP-1, RANTES and CXC/KC were confirmed by quantitative methods. These results suggest that ligands for TLR-4 stimulate both adipocytes and macrophages to up-regulate the expressions of many genes associated with inflammation and/or angiogenesis. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Nature Publishing Group |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/ijo.2008.153 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1038/ijo.2008.153 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0307-0565 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00680691 |
| 開始ページ |
|
|
開始ページ |
1725 |
| 書誌情報 |
International Journal of Obesity
International Journal of Obesity
巻 32,
号 11,
p. 1725-1729,
発行日 2008
|
| 旧ID |
25914 |
IntJObes_32_1725.pdf (106.9 KB)
