| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
CD133(+) cells from human umbilical cord blood reduce cortical damage and promote axonal growth in neonatal rat organ co-cultures exposed to hypoxia |
|
言語 |
en |
| 作成者 |
Tanaka, Norifumi
Kamei, Naosuke
Nakamae, Toshio
Yamamoto, Risako
Ishikawa, Masakazu
Fujiwara, Hisaya
Miyoshi, Hiroshi
Asahara, Takayuki
Ochi, Mitsuo
Kudo, Yoshiki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2010 ISDN Published by Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Brain damage |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
CD133 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Hypoxia |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Neonatal rat |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Slice culture |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
To evaluate the effect of CD133(+) cells (endothelial progenitor cells) on the hypoxia-induced suppression of axonal growth of cortical neurons and the destruction of blood vessels (endothelial cells), we used anterograde axonal tracing and immunofluorescence in organ co-cultures of the cortex and the spinal cord from 3-day-old neonatal rats. CD133(+) cells prepared from human umbilical cord blood were added to the organ co-cultures after hypoxic insult, and axonal growth, vascular damage and apoptosis were evaluated. Anterograde axonal tracing with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate was used to analyze axonal projections from the cortex to the spinal cord. Immunolabeling co-cultured tissues of the cortex and the spinal cord were used to investigate the effect of CD133(+). cells on the survival of blood vessels and apoptosis in the brain cortex. Hypoxia remarkably suppressed axonal growth in organ co-cultures of the cortex and the spinal cord, and this suppression was significantly restored by the addition of CD133(+). cells. CD133(+) cells also reduced the hypoxia-induced destruction of the cortical blood vessels and apoptosis. CD133(+) cells had protective effects on hypoxia-induced injury of neurons and blood vessels of the brain cortex in vitro. These results suggest that CD133(+) cell transplantation may be a possible therapeutic intervention for perinatal hypoxia-induced brain injury. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Pergamon Elsevier Science Ltd |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.ijdevneu.2010.07.232 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.ijdevneu.2010.07.232 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0736-5748 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10637655 |
| 開始ページ |
|
|
開始ページ |
581 |
| 書誌情報 |
International Journal of Developmental Neuroscience
International Journal of Developmental Neuroscience
巻 28,
号 7,
p. 581-587,
発行日 2010-11
|
| 旧ID |
30800 |
IntJDevNeurosci_28_581.pdf (1.9 MB)
