| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Biogenesis of Actinobacillus actinomycetemcomitans Cdt holotoxin |
|
言語 |
en |
| 作成者 |
Ueno, Yoko
Ohara, Masaru
Kawamoto, Toru
Fujiwara, Tamaki
Komatsuzawa, Hitoshi
Oswald, Eric
Sugai, Motoyuki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 American Society for Microbiology. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cdt |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
lipoprotein |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Actinobacillus actinomycetemcomitan |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
The cell cycle G2/M specific inhibitor, Cytolethal Distending Toxin (Cdt), from Actinobacillus actinomycetemcomitans is composed of CdtA, CdtB, and CdtC coded on the CdtA, CdtB and CdtC genes that are tandem on the chromosomal cdt locus. A. actinomycetemcomitans CdtA has the lipid binding consensus domain, the so-called “lipobox", at the N-terminal signal sequence. Using E.coli carrying pTK3022, we show the sixteenth residue, cysteine, of CdtA bound [3H]palmitate or [3H]glycerol. Further post translational processing of the signal peptide, CdtA, was inhibited using globomycin, an inhibitor of lipoprotein-specific signal peptidase II. Fractionation and immunoblot show the lipid modified CdtA is present in the outer membrane. Immuno-precipitation and the pull-down assay of the Cdt complex from E. coli carrying a plasmid containing CdtABC demonstrated the Cdt complex in the periplasm is composed of CdtA, CdtB, and CdtC, and that the Cdt complex in culture supernatant is a N-terminally truncated (36-43 amino acids) form of CdtA (CdtA'), CdtB, and CdtC. This suggests Cdt is present as a complex both in the periplasm and the supernatant where CdtA undergoes post-translation processing to CdtA' in the process of biogenesis and secretion of Cdt holotoxin into the culture supernatant. Site-directed mutagenesis of the sixteenth cysteine residue to glycine in CdtA altered localization of CdtA in the cell and reduced the amount of Cdt activity in the culture supernatant. This suggests Cdt forms a complex inside the periplasm for lipid modification where post-translational processing of CdtA plays an important role for the efficient production of Cdt holotoxin into the culture supernatant. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1128/IAI.00739-05 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
16714579 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1128/IAI.00739-05 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0019-9567 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00673732 |
| 開始ページ |
|
|
開始ページ |
3480 |
| 書誌情報 |
Infection and Immunity
Infection and Immunity
巻 74,
号 6,
p. 3480-3487,
発行日 2006-06
|
| 旧ID |
17081 |
IAI_74_3480.pdf (357.3 KB)
