| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Eldecalcitol (ED-71)-induced exosomal miR-6887-5p suppresses squamous cell carcinoma cell growth by targeting heparin-binding protein 17/fibroblast growth factor–binding protein-1 (HBp17/FGFBP-1) |
|
言語 |
en |
| 作成者 |
Higaki, M.
Shintani, T.
Hamada, A.
Rosli, S. N. Z.
Okamoto, T.
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| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
This is a post-peer-review, pre-copyedit version of an article published in In Vitro Cellular and Developmental Biology - Animal. The final authenticated version is available online at: https://doi.org/10.1007/s11626-020-00440-x |
| 権利情報 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
miR-6887-5p |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Eldecalcitol (ED-71) |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
HBp17/FGFBP-1 |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Oral squamous cell carcinoma |
| 主題 |
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主題Scheme |
Other |
|
主題 |
Exosome |
| 内容記述 |
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|
内容記述 |
Heparin-binding protein 17/fibroblast growth factor–binding protein-1 (HBp17/FGFBP-1) was purified from A431 cell-conditioned media based on its capacity to bind to fibroblast growth factor 1 and 2 (FGF-1 and FGF-2). HBp17/FGFBP-1 has been observed to induce the tumorigenic potential of epithelial cells and is highly expressed in oral cancer cell lines and tissues. HBp17/FGFBP-1 is also recognized as a pro-angiogenic molecule as a consequence of its interaction with FGF-2. We have previously reported that Eldecalcitol (ED-71), an analog of 1α,25(OH)2D3, downregulated the expression of HBp17/FGFBP-1 and inhibited the proliferation of squamous cell carcinoma (SCC) cells in vitro and in vivo through NF-κb inhibition. To explore the possibility of microRNA (miRNA) control of HBp17/FGFBP-1, we analyzed exosomal miRNAs from medium conditioned by A431 cells treated with ED-71. Microarray analysis revealed that 12 exosomal miRNAs were upregulated in ED-71-treated A431 cells. Among them, miR-6887-5p was identified to have a predicted mRNA target matching the 3′ untranslated region (3′-UTR) of HBp17/FGFBP-1. The 3′-UTR of HBp17/FGFBP-1 was confirmed to be a direct target of miR-6887-5p in SCC/OSCC cells, as assessed with a luciferase reporter assay. Functional assessment revealed that overexpression of miR-6887-5p in SCC/OSCC cells inhibited cell proliferation and colony formation in vitro, and inhibited tumor growth in vivo compared with control. In conclusion, our present study supports a novel anti-cancer mechanism involving the regulation of HBp17/FGFBP-1 function by exosomal miR-6887-5p in SCC/OSCC cells, which has potential utility as a miRNA-based cancer therapy. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This research was supported in part by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology to T.O. (grant number: 15H05043) and T. S. (grant number: 16K11723). |
| 出版者 |
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|
出版者 |
The Society for In Vitro Biology |
| 出版者 |
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|
出版者 |
Springer Verlag |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1007/s11626-020-00440-x |
| 関連情報 |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
32185608 |
| 関連情報 |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s11626-020-00440-x |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1071-2690 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1543-706X |
| 開始ページ |
|
|
開始ページ |
222 |
| 書誌情報 |
In Vitro Cellular and Developmental Biology - Animal
In Vitro Cellular and Developmental Biology - Animal
巻 56,
号 3,
p. 222-233,
発行日 2020-03-17
|
| 旧ID |
51080 |
InVitroCellDevBiolAnim_56_222.pdf (2.6 MB)
