| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Generation of hybrid hepatocytes by cell fusion from monkey embryoid body cells in the injured mouse liver |
|
言語 |
en |
| 作成者 |
Okamura, Kentaro
Asahina, Kinji
Fujimori, Hiroaki
Ozaki, Rie
Shimizu-Saito, Keiko
Tanaka, Yujiro
Teramoto, Kenichi
Arii, Shigeki
Takase, Kozo
Kataoka, Miho
Soeno, Yoshinori
Tateno, Chie
Yoshizato, Katsutoshi
Teraoka, Hirofumi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2005 Springer-Verlag |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
cynomolgus |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
embryonic stem cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
hepatocyte differentiation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
transgenic mouse |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
xenogeneic transplantation |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Monkey embryonic stem (ES) cells have characteristics that are similar to human ES cells, and might be useful as a substitute model for preclinical research. When embryoid bodies (EBs) formed from monkey ES cells were cultured, expression of many hepatocyte-related genes including cytochrome P450 (Cyp) 3a and Cyp7a1 was observed. Hepatocytes were immunocytochemically observed using antibodies against albumin (ALB), cytokeratin-8/18, and α1-antitrypsin in the developing EBs. The in vitro differentiation potential of monkey ES cells into the hepatic lineage prompted us to examine the transplantabilityof monkey EB cells. As an initial approach to assess the repopulation potential, we transplanted EB cells into immunodeficient urokinase-type plasminogen activator transgenic mice that undergo liver failure. After transplantation, the hepatocyte colonies expressing monkey ALB were observed in the mouse liver. Fluorescence in-situ hybridization revealed that the repopulating hepatocytes arise from cell fusion between transplanted monkey EB cells and recipient mouse hepatocytes. In contrast, neither cell fusion nor repopulation of hepatocytes was observed in the recipient liver after undifferentiated ES cell transplantation. These results indicate that the differentiated cells in developing monkey EBs, but not contaminating ES cells, generate functional hepatocytes by cell fusion with recipient mouse hepatocytes, and repopulate injured mouse liver. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Springer-Verlag |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
関連名称 |
The original version is abailable at www.springerlink.com |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s00418-005-0065-1 |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
16195892 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1007/s00418-005-0065-1 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11033302 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0948-6143 |
| 開始ページ |
|
|
開始ページ |
247 |
| 書誌情報 |
Histochemistry and Cell Biology
Histochemistry and Cell Biology
巻 125,
号 3,
p. 247-257,
発行日 2006-03
|
| 旧ID |
23544 |
HCB_125_247.pdf (725.0 KB)
