{"created":"2025-02-21T03:35:41.302353+00:00","id":2006912,"links":{},"metadata":{"_buckets":{"deposit":"afdebac9-5ba0-410e-904a-70c2efd880c1"},"_deposit":{"created_by":41,"id":"2006912","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2006912"},"status":"published"},"_oai":{"id":"oai:hiroshima.repo.nii.ac.jp:02006912","sets":["1730444907710"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/ Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yukawa, Masashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yukawa","familyNameLang":"en"}],"givenNames":[{"givenName":"Masashi","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamada, Yusuke","creatorNameLang":"en"}],"familyNames":[{"familyName":"Yamada","familyNameLang":"en"}],"givenNames":[{"givenName":"Yusuke","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"Toda, Takashi","creatorNameLang":"en"}],"familyNames":[{"familyName":"Toda","familyNameLang":"en"}],"givenNames":[{"givenName":"Takashi","givenNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"Copyright © 2019 Yukawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/ licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"fission yeast","subitem_subject_scheme":"Other"},{"subitem_subject":"kinesin","subitem_subject_scheme":"Other"},{"subitem_subject":"microtubule dynamics","subitem_subject_scheme":"Other"},{"subitem_subject":"mitotic spindle","subitem_subject_scheme":"Other"},{"subitem_subject":"suppressor","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The Kinesin-5 motor Cut7 in Schizosaccharomyces pombe plays essential roles in spindle pole separation, leading to the assembly of bipolar spindle. In many organisms, simultaneous inactivation of Kinesin-14s neutralizes Kinesin-5 deficiency. To uncover the molecular network that counteracts Kinesin-5, we have conducted a genetic screening for suppressors that rescue the cut7-22 temperature sensitive mutation, and identified 10 loci. Next generation sequencing analysis reveals that causative mutations are mapped in genes encoding a-, b-tubulins and the microtubule plus-end tracking protein Mal3/EB1, in addition to the components of the Pkl1/Kinesin-14 complex. Moreover, the deletion of various genes required for microtubule nucleation/polymerization also suppresses the cut7 mutant. Intriguingly, Klp2/ Kinesin-14 levels on the spindles are significantly increased in cut7 mutants, whereas these increases are negated by suppressors, which may explain the suppression by these mutations/deletions. Consistent with this notion, mild overproduction of Klp2 in these double mutant cells confers temperature sensitivity. Surprisingly, treatment with a microtubule-destabilizing drug not only suppresses cut7 temperature sensitivity but also rescues the lethality resulting from the deletion of cut7, though a single klp2 deletion per se cannot compensate for the loss of Cut7. We propose that microtubule assembly and/or dynamics antagonize Cut7 functions, and that the orchestration between these two factors is crucial for bipolar spindle assembly.","subitem_description_language":"en"},{"subitem_description":"This work was supported by the Japan Society for the Promotion of Science (JSPS) [KAKENHI Scientific Research (A) (16H02503 to T.T.), a Challenging Exploratory Research grant (16K14672 to T.T.), Scientific Research (C) (16K07694 to M.Y.)], the Naito Foundation (T.T.) and the Uehara Memorial Foundation (T.T). M.Y. and T.T. designed research.M.Y. and Y.Y. performed experiments and analyzed the data.","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Genetics Society of America"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"2160-1836","subitem_source_identifier_type":"ISSN"}]},"item_1617187024783":{"attribute_name":"Page Start","attribute_value_mlt":[{"subitem_start_page":"269"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"280","bibliographicPageStart":"269","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"G3:Genes, Genomes, Genetics"},{"bibliographic_title":"G3:Genes, Genomes, GeneticsG3"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1534/g3.118.200896","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1534/g3.118.200896","subitem_relation_type_select":"DOI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-18"}],"displaytype":"simple","filename":"G3_9_269.pdf","filesize":[{"value":"2.1 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://hiroshima.repo.nii.ac.jp/record/2006912/files/G3_9_269.pdf"},"version_id":"6846020e-08c3-4e7f-bf77-20aab572ec22"}]},"item_1732771732025":{"attribute_name":"旧ID","attribute_value":"48609"},"item_title":"Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/ Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe","item_type_id":"40003","owner":"41","path":["1730444907710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-18"},"publish_date":"2023-03-18","publish_status":"0","recid":"2006912","relation_version_is_last":true,"title":["Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/ Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2025-02-21T08:44:04.707075+00:00"}