| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Direct inhibition of Indian hedgehog expression by parathyroid hormone (PTH)/PTH-related peptide and up-regulation by retinoic acid in growth plate chondrocyte cultures |
|
言語 |
en |
| 作成者 |
Yoshida, Eri
Noshiro, Mitsuhide
Kawamoto, Takeshi
Tsutsumi, Shinichi
Kuruta, Yoshihiro
Kato, Yukio
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2001 Academic Press. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
PTH |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
PTHrP |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
RA |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ihh |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
chondrocyte |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
growth plate |
| 内容記述 |
|
|
内容記述 |
Indian hedgehog (Ihh) is highly expressed in prehypertrophic chondrocytes in vivo and has been proposed to regulate the proliferation and maturation of chondrocytes and bone collar formation in the growth plate. In high-density cultures of rabbit growth-plate chondrocytes, Ihh mRNA was also expressed at the highest level in the prehypertrophic stage. To explore endogenous factors that regulate Ihh expression in chondrocytes, we examined the effects of various growth factors on Ihh mRNA expression in this system. Retinoic acid (RA) and bone morphogenetic protein-2 enhanced Ihh mRNA expression, whereas PTH/PTH-related peptide (PTHrP) markedly suppressed Ihh expression. RA at more than 10-8 M induced the expression of Ihh and Patched 1 (Ptc1) within 3 h, before it increased the type X collagen mRNA level at 6–24 h. Cycloheximide blocked the up-regulation of Ihh by RA, indicating the requirement of de novo protein synthesis for this stimulation. These findings suggest that RA is involved in the up-regulation of Ihh during endochondral bone formation. In contrast to RA, PTH (1–84) at 10-7 M abolished the mRNA expression of Ihh and Ptc1 within 2–4 h, before it suppressed the expression of type X collagen at 12–24 h. The inhibition of Ihh expression by PTH (1–84) did not require de novo protein synthesis. PTH (1–34), PTHrP (1–34), and (Bu)2cAMP also suppressed Ihh expression. On the other hand, Ihh has been reported to induce PTHrP synthesis in the perichondrium. Consequently, the direct inhibitory action of PTH/PTHrP on Ihh appears to be a negative feedback mechanism that prevents excess PTHrP accumulation in cartilage. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1006/excr.2001.5161 |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
11281644 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1006/excr.2001.5161 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0014-4827 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00641011 |
| 開始ページ |
|
|
開始ページ |
64 |
| 書誌情報 |
Experimental Cell Research
Experimental Cell Research
巻 265,
号 1,
p. 64-72,
発行日 2001-04-15
|
| 旧ID |
20100 |
ExpCellRes_265-1_64.pdf (1016.6 KB)
