| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
A second isoform of 3-ketoacyl-CoA thiolase found in Caenorhabditis elegans, which is similar to sterol carrier protein x but lacks the sequence of sterol carrier protein 2 |
|
言語 |
en |
| 作成者 |
Bun-ya, Masanori
Maebuchi, Motohiro
Hashimoto, Takeshi
Yokota, Sadaki
Kamiryo, Tatsuyuki
|
| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
Copyright (c) 1999 Federation of European Biochemical Societies |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
cDNA cloning |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
3-ketoacyl-CoA thiolase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
sterol carrier protein x |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
sterol carrier protein 2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Caenorhabditis elegans |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
We cloned a full-length cDNA of the nematode Caenorhabditis elegans that encodes a 44-kDa protein (P-44, 412 residues) similar to sterol carrier protein x (SCPx). Mammalian SCPx is a bipartite protein: its 404-residue N-terminal and 143-residue C-terminal domains are similar to 3-ketoacyl-CoA thiolase and identical to the precursor of sterol carrier protein 2 (SCP2; also termed non-specific lipid-transfer protein), respectively. P-44 has 56(null)equence identity to the thiolase domain of SCx but lacks the SCP2 sequence. Northern blot analysis revealed only a single mRNA species of 1.4 kb, which agrees well with the length of the cDNA (1371 bp), making it improbable that alternative splicing produces an SCPx-like fusion protein. The sequence similarities of P-44 to conventional thiolases are lesser than that to SCPx. Purified recombinant P-44 cleaved long-chain 3-ketoacyl-CoAs (C8-16) in a thiolytic manner by the ping-pong bi-bi reaction mechanism. The inhibition of P-44 by acetyl-CoA was competitive with CoA and non-competitive with 3-ketooctanoyl-CoA. This pattern of inhibition is shared with SCPx but not with conventional 3-ketoacyl-CoA thiolase, which is inhibited uncompetitively with respect to 3-ketoacyl CoA. From these results, we concluded that nematode P-44 and mammalian SCPx constitute a second isoform of thiolase, which we propose to term type-II 3-ketoacyl-CoA thiolase. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Federation of European Biochemical Societies |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
9151950 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0014-2956 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00639541 |
| 開始ページ |
|
|
開始ページ |
252 |
| 書誌情報 |
European Journal of Biochemistry
European Journal of Biochemistry
巻 245,
号 2,
p. 252-259,
発行日 1997
|
| 旧ID |
14887 |
ejb_245_252.pdf (1.4 MB)
