| アイテムタイプ |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
The variable region of RNA2 is sufficient to determine host specificity in betanodaviruses. |
|
言語 |
en |
| 作成者 |
Ito, Yu
Okinaka, Yasushi
Mori, Koh-Ichiro
Sugaya, Takuma
Nishioka, Toyohiro
Oka, Masakazu
Nakai, Toshihiro
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2008 Inter-Research. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Betanodavirus |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Host specificity |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Viral nervous necrosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Chimeric virus |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
RNA2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
T4 region |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
SJNNV |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
RGNNV |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
480 |
| 内容記述 |
|
|
内容記述 |
Betanodaviruses, the causative agents of viral nervous necrosis in marine fish, have bipartite positive-sense RNA genomes. The viruses have been classified into 4 distinct types based on nucleotide sequence similarities in the variable region (the so-called T4 region) of the smaller genomic segment RNA2 (1.4 kb). Betanodaviruses have marked host specificity, although the primary structures of the viral RNAs and encoded proteins are similar among the viruses. We have previously demonstrated, using reassortants between striped jack nervous necrosis virus (SJNNV) and redspotted grouper nervous necrosis virus (RGNNV), that RNA2, which encodes the coat protein, strictly controls host specificity. However, because RNA2 is large, we were unable to propose a mechanism underlying this RNA2-based host specificity. To identify the RNA2 region that controls host specificity, we constructed RNA2 chimeric viruses from SJNNV and RGNNV and tested their infectivity in the original host fish, striped jack Pseudocaranx dentex and sevenband grouper Epinephelus septemfasciatus. Among these chimeric viruses, SJNNV mutants containing the variable region of RGNNV RNA2 infected sevenband grouper larvae in a manner similar to RGNNV, while RGNNV mutants containing the variable region of SJNNV RNA2 infected striped jack larvae in a manner similar to SJNNV. Immunofluorescence microscopic studies using anti-SJNNV polyclonal antibodies revealed that these chimeric viruses multiplied in the brains, spinal cords and retinas of the infected fish, as in infections by the parental viruses. These results indicate that the variable region of RNA2 is sufficient to control host specificity in SJNNV and RGNNV. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Inter-Research |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3354/dao01906 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.3354/dao01906 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0177-5103 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1616-1580 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10443976 |
| 開始ページ |
|
|
開始ページ |
199 |
| 書誌情報 |
Diseases of Aquatic Organisms
Diseases of Aquatic Organisms
巻 79,
号 3,
p. 199-205,
発行日 2008-05-08
|
| 旧ID |
25665 |
dao079p199.pdf (927.7 KB)
