| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Cloning of the fish cell line SSN-1 for piscine nodaviruses. |
|
言語 |
en |
| 作成者 |
Iwamoto, Tokinori
Nakai, Toshihiro
Mori, Koh-ichiro
Arimoto, Misao
Furusawa, Iwao
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2000 Inter-Research. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Nodavirus |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Viral nervous necrosis, VNN |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
SSN-1 cell line |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cell cloning |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
C-type retrovirus |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Snakehead retrovirus |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
480 |
| 内容記述 |
|
|
内容記述 |
Six cell clones were derived from the SSN-1 cell line, which is composed of a mixed cell population and persistently infected with a C-type retrovirus (SnRV). These clones were susceptible to 4 piscine nodavirus strains belonging to different genotypes (SJNNV, RGNNV, TPNNV and BFNNV [striped jack, redspotted grouper, tiger puffer and barfin flounder nervous necrosis viruses]). Three clones, designated A-6, E-9, and E-11, were highly permissive to nodavirus infection and production. The virus-induced cytopathic effects appeared as cytoplasmic vacuoles and intensive disintegration at 3 to 5 d post-incubation. These observations were highly reproducible and formed the basis for a successful virus titration system. Quantitative analysis using the cloned E-11 cell line clearly revealed differences in the optimal growth temperatures among the 4 genotypic variants: 25 to 30°C for strain SGWak97 (RGNNV), 20 to 25°C for strain SJNag93 (SJNNV), 20°C for strain TPKag93 (TPNNV), and 15 to 20°C for strain JFIwa98 (BFNNV). Electron microscopy demonstrated SnRV retrovirus particles only in A-6 and E-9 cells, but PCR amplification for the pol gene and LTR region of the proviral DNA indicated the presence of the retrovirus in the other clones, including E 11. The cell clones obtained in the present study will be more useful for qualitative and quantitative analyses of piscine nodaviruses than the SSN-1 cell line. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Inter-Research |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3354/dao043081 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.3354/dao043081 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0177-5103 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1616-1580 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10443976 |
| 開始ページ |
|
|
開始ページ |
81 |
| 書誌情報 |
Diseases of Aquatic Organisms
Diseases of Aquatic Organisms
巻 43,
号 2,
p. 81-89,
発行日 2000-11-14
|
| 旧ID |
25660 |
dao043p081.pdf (740.1 KB)
