| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis (SJNNV) |
|
言語 |
en |
| 作成者 |
Nishizawa, Toyohiko
Mori, Koh-ichiro
Nakai, Toshihiro
Furusawa, Iwao
Muroga, Kiyokuni
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 1994 Inter-Research. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Viral nervous necrosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Nodavirus |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
PCR |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
480 |
| 内容記述 |
|
|
内容記述 |
The polymerase chain reaction (PCR) was used to amplify a portion of the coat protein gene (RNA2) of striped jack nervous necrosis vlrus (SJNNV), the causative agent of viral nervous necrosis (VNN) of larval striped jack Pseudocaranx dentex. Based on the sequence data of SJNNV RNA2, 2 forward (F1 and F2) and 3 reverse (RI. R2 and R3) PCR primers were synthesized and the 5 potential target regions were amplified with a combination of these primers. After reverse transcription of genomic RNA extracted from SJNNV and 25 cycles of PCR amplification, products of the expected size were detected separately on agarose gels stained with ethidium bromide. Southern blot hybridization confirmed that all of the amplified products were specific to cDNA of SJNNV RNA2. Two primer sets, F1-R2 and F2-R3, produced the specified 180 bp and 430 bp products. The PCR system, using the F2-R3 primer set, was able to detect 100 fg of SJNNV RNA after 25 cycles and was also able to efficiently amplify the target region of SJNNV gene in the total nucleic acids extracted from larval striped jack affected with VNN. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Inter-Research |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3354/dao018103 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.3354/dao018103 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0177-5103 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1616-1580 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10443976 |
| 開始ページ |
|
|
開始ページ |
103 |
| 書誌情報 |
Diseases of Aquatic Organisms
Diseases of Aquatic Organisms
巻 18,
p. 103-107,
発行日 1994-02-24
|
| 旧ID |
25651 |
dao018p103.pdf (530.3 KB)
